(KO Validated) Caspase-3 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1580 / ￥2520 / ￥4020 |
货号：E-AB-60646
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | CPP32,CPP32B,SCA-1,Active Caspase 3,CASP3,active Caspase-3,Caspase 3,Caspase-3 p12,caspase-3,CASP3抗体 |
| Swissprot | P42574 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant fusion protein of human Caspase-3 (NP_004337.2). |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,, 1:500-1:2000;IHC,, 1:50-1:200; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Background | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. |
Images
Western blot analysis of extracts of Jurkat cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts from normal (control) and Caspase-3 knockout (KO) 293T cells using Caspase-3 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat spleen using Caspase-3 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human tonsil using Caspase-3 Polyclonal Antibody at dilution of 1:100 (40x lens).
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