ACTN4 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-14759
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | actinin 4,Actinin alpha 4,actinin4,ACTN 4,ACTN4,ACTN4,Alpha-actinin-4,DKFZp686K23158,F actin cross linking protein,F-actin cross-linking protein,Focal segmental glomerulosclerosis 1,FSGS 1,FSGS,FSGS1,Non muscle alpha actinin 4,Non-muscle alpha-actinin 4,ACTN4抗体 |
| Swissprot | O43707 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human ACTN4 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:50-1:200; |
| Concentration | 0.8 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines. |
| Tissue specificity | Widely expressed. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. |
Images
Western Blot analysis of Lovo cell and Mouse kidney tissue, 231 and hepG2 cell using ACTN4 Polyclonal Antibody at dilution of 1:485
Immunohistochemistry of paraffin-embedded Human esophagus cancer using ACTN4 Polyclonal Antibody at dilution of 1:60
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ACTN4 Polyclonal Antibody at dilution of 1:60
