Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP Method)
碱性磷酸酶(ALP)比色法测试盒(对硝基苯磷酸二钠法)
| 规格： | / 48T(31 samples) / 96T(79 samples) |
| 价格： | / ￥260 / ￥320 |
货号：E-BC-K009-M
检测仪器及波长: 酶标仪(400-415 nm，最佳检测波长405 nm)
样本类型: 动物组织样本;血清(浆)等液体样本与细胞样本
检测原理
在碱性条件下，碱性磷酸酶催化对硝基苯磷酸二钠水解，生成对硝基苯酚和磷酸。对硝基苯酚在强碱性条件下呈亮黄色，在405 nm处有强烈的吸收峰。因此可以测定405 nm 处的吸收值来计算产物的生成量，从而计算出酶活。
关键信息
| 指标简称 | ALP |
| 样本类型 | 动物组织样本;血清(浆)等液体样本与细胞样本 |
| 灵敏度 | 0.27 U/L |
| 检测范围 | 0.27-50.8 U/L |
| 精密度 | 批间差：4.6%批内差：1.2% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | -20℃ |
| 有效期 | 12个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围(0.27-50.8 U/L)，请参考下表稀释(仅供参考)：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
10-20 |
10%大鼠肾组织 |
400-1200 |
|
小鼠血清 |
20-30 |
10%小鼠肝组织 |
10-15 |
|
大鼠血清 |
10-20 |
10%大鼠肺组织 |
50-100 |
|
小鼠血浆 |
10-20 |
10%小鼠脑组织 |
30-50 |
|
HepG2 细胞上清 |
8-12 |
人尿液 |
不稀释 |
注：稀释液为试剂一。
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