alpha Tubulin Polyclonal Antibody
规格: | / 30μL / 60μL / 120μL / 300μL / 500μL |
价格: | / ¥295 / ¥548 / ¥875 / ¥1975 / ¥2835 |
货号:E-AB-20069
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC-p,IF,ELISA
Overview
Synonyms | Alpha tubulin 3,Alpha-tubulin 3,B alpha 1,FLJ25113,LIS3,TBA1A,TUBA1A,TUBA3,Tubulin alpha 1a,Tubulin alpha 1A chain,Tubulin alpha 3,Tubulin alpha 3 chain,Tubulin alpha brain specific,Tubulin alpha-1A chain,Tubulin alpha-3 chain,Tubulin B alpha 1,Tubulin B-alpha-1,Tubulin α抗体 |
Swissprot | Q71U36,P68363 |
Source | Rabbit |
Reactivity | Human,Mouse,Rat |
Immunogen | Synthesized peptide derived from the C-terminal region of human Tubulin α |
Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF,ELISA |
Recommended dilution | WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000 |
Concentration | 1mg/mL |
Clonality | Polyclonal |
Properties
Cellular localization | Cytoskeleton,cytoplasmic microtubule,microtubule,microtubule cytoskeleton,Cytosol,Endosome,recycling endosome,Extracellular region or secreted,extracellular exosome,Nucleus,Other locations:cytoplasmic ribonucleoprotein granule,membrane raft,myelin sheath |
Isotype | IgG |
Purification | Affinity purification |
Conjugation | Unconjugated |
Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
Background | There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. |
Images
Western Blot analysis of Hela, A549 cells using alpha Tubulin Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using alpha Tubulin Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human lung tissue using alpha Tubulin Polyclonal Antibody at dilution of 1:200.