Aspartate Aminotransferase (AST/GOT) Activity Assay Kit
谷草转氨酶(AST/GOT) 比色法测试盒
| 规格： | / 100 assays(45 samples) |
| 价格： | / ￥320 |
货号：E-BC-K236-S
检测仪器及波长: 紫外-可见光分光光度计(505 nm)
样本类型: 血清(浆);动物组织;细胞等样本
检测原理
谷草转氨酶(AST)催化天冬氨酸与α-酮戊二酸之间的氨基转换反应，生成谷氨酸和草酰乙酸，草酰乙酸在反应过程中可自行脱羧成丙酮酸。丙酮酸与苯肼反应，生成苯腙，苯腙在碱性条件下呈红棕色。
关键信息
| 指标简称 | AST,GOT |
| 样本类型 | 血清(浆);动物组织;细胞等样本 |
| 灵敏度 | 0.38 IU/L |
| 检测范围 | 0.38-72.30 IU/L |
| 精密度 | 批间差：4.9%批内差：4.2% |
| 检测仪器及波长 | 分光光度计 |
| 保存条件 | 2-8℃ |
| 有效期 | 12个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围(0.38-72.30 IU/L)，请参考下表稀释:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
大鼠血清 |
不稀释 |
|
人血浆 |
不稀释 |
小鼠血浆 |
不稀释 |
|
10%大鼠肝脏组织 |
16-32 |
10%大鼠肾脏组织 |
不稀释 |
|
肝细胞上清 |
不稀释 |
10%大鼠心组织 |
不稀释 |
注：稀释液用生理盐水
客户发表文献
- 文献: MicroRNA 15a/16‐1 suppresses aryl hydrocarbon receptor–dependent interleukin‐22 secretion in CD4+ T cells and contributes to immune‐mediated organ injury
样品类型: serum - 文献: Lipid Metabolic Disorder Induced by Pyrethroids in Nonalcoholic Fatty Liver Disease of Xenopus laevis
样品类型: plasma - 文献: The combined adverse effects of cis-bifenthrin and graphene oxide on lipid homeostasis in Xenopus laevis
- 文献: Synergistic Multimodal Cancer Therapy Using Glucose Oxidase@CuS Nanocomposites
样品类型: plasma - 文献: Human hepatocyte PNPLA3-148M exacerbates rapid non-alcoholic fatty liver disease development in chimeric mice
样品类型: serum - 文献: Multifunctional Graphene-Oxide-Reinforced Dissolvable Polymeric Microneedles for Transdermal Drug Delivery
样品类型: blood - 文献: Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal
样品类型: serum - 文献: An efficient and safe MUC1-dendritic cell-derived exosome conjugate vaccine elicits potent cellular and humoral immunity and tumor inhibition in vivo
样品类型: serum - 文献: The Citrus Flavonoid Nobiletin Downregulates Angiopoietin-like Protein 3 (ANGPTL3) Expression and Exhibits Lipid-Modulating Effects in Hepatic Cells and Adult Zebrafish Models
样品类型: blood - 文献: Activation of α7nAChR preserves intestinal barrier integrity by enhancing the HO-1 / STAT3 signaling to inhibit NF-κB activation in mice
样品类型: Serum - 文献: Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice
样品类型: serum - 文献: Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota
样品类型: serum - 文献: Glabridin inhibits liver fibrosis and hepatic stellate cells activation through suppression of inflammation and oxidative stress by activating PPARγ in carbon tetrachloride-treated mice
样品类型: serum - 文献: Atorvastatin Ester Regulates Lipid Metabolism in Hyperlipidemia Rats via the PPAR-signaling Pathway and HMGCR Expression in the Liver
- 文献: Fatty acids of Pleurotus florida mushroom: Potential molecules for blood glucose control
样品类型: blood - 文献: Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats
样品类型: serum - 文献: Identification of Chemical Compounds from Artemisia gmelinii using UPLC-QTOF-MS/MS and their Regulatory Effects on Immune Responses in DSS-Induced Colitis Mice
样品类型: serum - 文献: Gal-3 Deficiency Suppresses Novosphyngobium aromaticivorans Inflammasome Activation and IL-17 Driven Autoimmune Cholangitis in Mice
样品类型: serum - 文献: Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis
样品类型: serum - 文献: L-Carnitine Alleviates the Myocardial Infarction and Left Ventricular Remodeling through Bax/Bcl-2 Signal Pathway
样品类型: plasma - 文献: Benefits on Hematological and Biochemical Parameters of a High-Intensity Interval Training Program for a Half-Marathon in Recreational Middle-Aged Women Runners
样品类型: plasma - 文献: Hepatoprotective and neuroprotective effect of taxifolin on hepatic encephalopathy in rats
样品类型: serum - 文献: Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues
样品类型: serum - 文献: San-Huang-Chai-Zhu Formula Ameliorates Liver Injury in Intrahepatic Cholestasis through Suppressing SIRT1/PGC-1α-Regulated Mitochondrial Oxidative Stress
样品类型: hepatic tissue,serum - 文献: 3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis
样品类型: plasma - 文献: β-Hydroxybutyrate upregulates FGF21 expression through inhibition of histone deacetylases in hepatocytes
样品类型: serum - 文献: Chemoprotective Effect of Decalactone on Hepatic Cancer via Diminishing the Inflammatory Response and Oxidative Stress
样品类型: liver - 文献: Effect of silymarin on blood coagulation profile and osmotic fragility in carbon tetrachloride induced hepatotoxicity in male Wistar rats
样品类型: plasma - 文献: Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects
样品类型: plasma
