ATG5 Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22149
宿主: Mouse
反应性: H,M,R
应用: WB,IHC-p
Overview
| Synonyms | APG 5,APG 5L,APG5,APG5 autophagy 5 like,APG5 like,APG5-like,APG5L,Apoptosis specific protein,Apoptosis-specific protein,ASP,ATG 5,Atg5,ATG5 autophagy related 5 homolog,ATG5,Autophagy protein 5,Autophagy related 5,hAPG5,Homolog of S Cerevisiae autophagy 5,OTTHUMP00000040507,ATG5 Mouse (3C7)抗体 |
| Swissprot | Q9H1Y0 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein of ATG5 |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-2000;IHC,,1:50-300; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. |
| Tissue specificity | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. |
| Clone No | Clone:5D3 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II. |
Images
Western Blot analysis of Hela cells using ATG5 Monoclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Rat testis tissue using ATG5 Monoclonal Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human ovarian carcinoma tissue using ATG5 Monoclonal Antibody at dilution of 1:200.
客户发表文献
- 文献: Niclosamide suppresses T‑cell acute lymphoblastic leukemia growth through activation of apoptosis and autophagy
样品类型: Jurkat cell,CCRF-CEM cell
