ATG5 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70289
宿主: Rabbit
反应性: H
应用: IHC
Overview
| Synonyms | APG 5,APG 5L,APG5,APG5 autophagy 5 like,APG5 like,APG5-like,APG5L,Apoptosis specific protein,Apoptosis-specific protein,ASP,ATG 5,Atg5,ATG5 autophagy related 5 homolog,ATG5,Autophagy protein 5,Autophagy related 5,hAPG5,Homolog of S Cerevisiae autophagy 5,OTTHUMP00000040507,ATG5抗体 |
| Swissprot | Q9H1Y0,Q99J83 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | KLH conjugated Synthetic peptide corresponding to mouse ATG5 |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:200-1:800 |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. |
| Tissue specificity | Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.Highest expression level in colonic mucosa. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | ATG5,also named as APG5L and ASP,belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process,possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process,occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection. |
