ATP Colorimetric Assay Kit
ATP含量比色法测试盒
| 规格： | / 50 assays(24 samples) / 100 assays(48 samples) |
| 价格： | / ￥380 / ￥700 |
货号：E-BC-K157-S
检测仪器及波长: 紫外-可见光分光光度计（636 nm）
样本类型: 组织样本
检测原理
肌酸激酶催化肌酸和 ATP 反应生成磷酸肌酸，通过比色法检测磷酸肌酸的含量，以此反应出ATP的含量。
关键信息
| 指标简称 | ATP |
| 样本类型 | 组织样本 |
| 灵敏度 | 0.01 mmol/L |
| 检测范围 | 0.01-1.5 mmol/L |
| 精密度 | 批间差：9.3%批内差：3.6% |
| 检测仪器及波长 | 紫外-可见光分光光度计 |
| 特殊仪器 | 100℃恒温水浴锅 |
| 保存条件 | Reagent 4 -20℃，其余2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择差异较大的2-3例样本稀释成不同浓度进行预实验，结合检测范围：0.01-1.5 mmol/L，不同样本的稀释如下表(仅供参考)：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
10%大鼠肌肉组织 |
2-3 |
10%大鼠肾脏组织 |
2-3 |
|
10%大鼠肝脏组织 |
2-3 |
10%大鼠肺组织 |
2-3 |
|
10%大鼠脑组织 |
2-3 |
|
|
注：稀释液为去离子水
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