Bcl-2 Polyclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40526
宿主: Rabbit
反应性: Z
应用: WB
Overview
| Synonyms | Bcl-2, PPP1R50, BCL2 |
| Swissprot | Q564A4 |
| Source | Rabbit |
| Reactivity | Zebrafish |
| Immunogen | Synthetic peptide corresponding to zebrafish BCL-2 conjugated to keyhole limpet haemocyanin. |
| Application | WB(Detection kit: E-IR-R304) |
| Recommended dilution | WB,,1:1000-2000; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane |
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Bcl-2 (B-cell lymphoma 2), is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inducing (pro-apoptotic) or inhibiting (anti-apoptotic) apoptosis.Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87. |
