beta actin Monoclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-48018
宿主: Mouse
反应性: H,M,R,Z
应用: WB,IF
Overview
| Synonyms | ACTB, Actin beta, Actx, Actin cytoplasmic 1, b actin, A26C1A |
| Swissprot | Q7ZVF9 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat,Zebrafish |
| Immunogen | Recombinant human Beta actin protein |
| Application | WB(Detection kit: E-IR-R304),IF |
| Recommended dilution | WB,,1:1000-2000;IF,,1:100-200; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm, Cytoskeleton, Nucleus |
| Isotype | IgG2a |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Beta-actin (ACTB/ACTB) is one of six different actin isoforms in humans. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Beta-actin gene is stably and constitutively expressed at high levels in most tissues and cells, and commonly used as a loading control for western blot and as a control for qPCR. |
Images
Western Blotting of cell/tissue lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Lane1: PANC-1 cell lysates, Lane2: 293F cell lysates, Lane3: Human placenta lysates, Lane4: Mouse SP2/0 celll ysates, Lane5: Mouse kidney lysates.
Western blotting of Zebrafish lysates with anti-Beta Actin monoclonal antibody at dilution of 1:2000.
Immunofluorescence analysis of 293 cells with anti-beta actin monoclonal antibody at 1:100 dilution.
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