beta Amyloid Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70168
宿主: Rabbit
反应性: M,R
应用: IHC
Overview
| Synonyms | A4 amyloid protein,A4,AAA,ABETA,ABPP,AD1,AICD-50,AICD-57,AICD-59,AID(50),AID(57),AID(59),Alzheimer disease amyloid protein,Amyloid beta (A4) precursor protein,Amyloid beta A4 protein,Amyloid beta protein,Amyloid intracellular domain 50,Amyloid intracellular domain 57,Amyloid intracellular domain 59,Amyloid precursor protein,APP,APPI,Beta amyloid peptide,Beta-amyloid precursor protein,Beta-APP40,Beta-APP42,C31,Cerebral vascular amyloid peptide,CTFgamma,CVAP,Gamma-CTF(50),Gamma-CTF(57),Gamma-CTF(59),peptidase nexin II,PN 2,PN II,PN-II,PN2,PreA4,Protease nexin II,Protease nexin-II,S-APP-alpha,S-APP-beta,beta Amyloid抗体 |
| Swissprot | P12023,P08592 |
| Source | Rabbit |
| Reactivity | Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse APP |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:200-1:800 |
| Concentration | 308 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Membrane. Membrane>clathrin-coated pit. Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). After alpha-secretase cleavage, soluble APP is released into the extracellular space and the C-terminal is internalized to endosomes and lysosomes. Some APP accumulates in secretory transport vesicles leaving the late Golgi compartment and returns to the cell surface. Gamma-CTF(59) peptide is located to both the cytoplasm and nuclei of neurons. It can be translocated to the nucleus through association with APBB1 (Fe65). Beta-APP42 associates with FRPL1 at the cell surface and the complex is then rapidly internalized. APP sorts to the basolateral surface in epithelial cells. During neuronal differentiation, the Thr-743 phosphorylated form is located mainly in growth cones, moderately in neurites and sparingly in the cell body. Casein kinase phosphorylation can occur either at the cell surface or within a post-Golgi compartment. Associates with GPC1 in perinuclear compartments. Colocalizes with SORL1 in a vesicular pattern in cytoplasm and perinuclear regions. |
| Tissue specificity | Expressed in the brain with expression in cortex, cerebellum, hippocampus, olfactory bulb, neurons, astrocytes and microglia (at protein level) . Isoform APP770 is expressed in kidney. Isoform APP751 is widely expressed. Isoform APP695 is expressed in brain, kidney and liver. Isoform APP695, isoform APP714 and isoform APP751 are expressed in several different brain regions including hippocampus, substania nigra pars compacta and cerebellum. In the cerebellum, these isoforms are abundantly expressed in Purkinje cells.Highest expression level in cingulate cortex. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Aβ derives from APP via proteolytic cleavage by proteases called α-,β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ,while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP),encoded by APP gene which locate on human chromosome 21q,is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth,neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain,kidney,heart and spleen,but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1). |
