BRCA1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-30669
宿主: Rabbit
反应性: H,R
应用: WB,IHC-p,IF
Overview
| Synonyms | BRCA 1,BRCA1,BRCA1 DNA repair associated,BRCA1/BRCA2 containing complex subunit 1,BRCA1/BRCA2-containing complex,subunit 1,BRCA1,BRCAI,BRCC 1,BRCC1,Breast and ovarian cancer susceptibility protein 1,Breast Cancer 1,Breast Cancer 1 Early Onset,Breast cancer type 1 susceptibility protein,BROVCA1,FANCS,IRIS,PNCA4,PPP1R53,Protein phosphatase 1 regulatory subunit 53,PSCP,RING finger protein 53,RNF53,BRCA1抗体 |
| Swissprot | P38398 |
| Source | Rabbit |
| Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from human BRCA1 around the non-phosphorylation site of Ser1423. |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300;IF,,1:200-1:1000; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex. |
| Tissue specificity | Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. |
Images
Western Blot analysis of 293T-UV, 22RV1 cells using BRCA1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human uterus cancer tissue using BRCA1 Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat lung tissue using BRCA1 Polyclonal Antibody at dilution of 1:200.
