CALR Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-10352
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | Autoantigen RO,CALR,CALR protein,CALR,Calregulin,Calreticulin,cC1qR,CRP55,CRT,CRTC,Endoplasmic reticulum resident protein 60,Epididymis secretory sperm binding protein Li 99n,ERp60,FLJ26680,grp60,HACBP,HEL S 99n,RO,Sicca syndrome antigen A (autoantigen Ro,calreticulin),Sicca syndrome antigen A,SSA,CALR抗体 |
| Swissprot | P27797 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human CALR |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:1000-1:5000;IHC,,1:50-1:200; |
| Concentration | 0.4 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Endoplasmic reticulum lumen. Cytoplasm>cytosol. Secreted>extracellular space>extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. |
Images
Western Blot analysis of NIH/3T3 cell using CALR Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human gastic cancer using CALR Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Human ovarian cancer using CALR Polyclonal Antibody at dilution of 1:50
客户发表文献
- 文献: Annexin A1 Released in Extracellular Vesicles by Pancreatic Cancer Cells Activates Components of the Tumor Microenvironment, through Interaction with the Formyl-Peptide Receptors
样品类型: extracellular vesicles，Cell lysate - 文献: Annexin A1 May Induce Pancreatic Cancer Progression as a Key Player of Extracellular Vesicles Effects as Evidenced in the In Vitro MIA PaCa-2 Model System
样品类型: Cell culture supernatant
