Caspase-4 Monoclonal Antibody(Capture)
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN001480P
货期：咨询
宿主: Rat
反应性: H
应用: ELISA Capture
Overview
| Synonyms | Caspase-4;CASP-4;ICE and Ced-3 homoLog 2;ICE(reL)-II;Mih1;Protease TX;CASP4;ICH2 |
| Swissprot | P49662 |
| Source | Rat |
| Reactivity | Human |
| Immunogen | Recombinant Human Caspase-4 protein expressed by E.coli |
| Application | ELISA Capture |
| Recommended dilution | ELISA Capture,,2-8 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | 3A9 |
|---|---|
| Isotype | IgG1 |
| Purification | Protein A/G Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (lPS)-induced pyroptosis .Also indirectly activates the NlRP3 and NlRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS,GSDMD and Il18 . Effector of the non-canonical inflammasome independently of NlRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine Il1B and Il18 . In the non-canonical inflammasome,CASP4 is activated by direct binding to lPS without the need of an upstream sensor . lPS-binding promotes CASP4 activation and CASP4-mediated cleavage of GSDMD,followed by pyroptosis of infected cells and their extrusion into the gut lumen . Also indirectly promotes secretion of mature cytokines (Il1A,Il18 and HMGB1) downstream of GSDMD-mediated pyroptosis via activation of the NlRP3 and NlRP6 inflammasomes . Involved in NlRP3-dependent CASP1 activation and Il1B and Il18 secretion in response to non-canonical activators,such as UVB radiation or cholera enterotoxin . Involved in NlRP6 inflammasome-dependent activation in response to lipoteichoic acid (lTA),a cell-wall component of Gram-positive bacteria,which leads to CASP1 activation and Il1B and Il18 secretion . Involved in lPS-induced Il6 secretion,this activity may not require caspase enzymatic activity. The non-canonical inflammasome is required for innate immunity to cytosolic,but not vacuolar,bacteria. Plays a crucial role in the restriction of S.typhimurium replication in colonic epithelial cells during infection. |
