Catenin beta Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22111
宿主: Mouse
反应性: H,M,R
应用: WB,IHC-p,IF
Overview
| Synonyms | Beta catenin,Beta-catenin,Cadherin associated protein,Catenin (cadherin associated protein),beta 1,88kDa,Catenin beta 1,Catenin beta-1,CATNB,CHBCAT,CTNB1,CTNNB,CTNNB1,DKFZp686D02253,FLJ25606,FLJ37923,OTTHUMP00000162082,OTTHUMP00000165222,OTTHUMP00000165223,OTTHUMP00000209288,OTTHUMP00000209289,Catenin-β抗体 |
| Swissprot | P35222 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Protein |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300;IF,,1:100-1:300; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. |
| Tissue specificity | Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. |
| Clone No | Clone:2E4 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. |
Images
Western Blot analysis of 1) Hela, 2) 293T, 3) Mouse liver, 4) Rat liver with Catenin beta Monoclonal Antibody
Immunohistochemistry of paraffin-embedded Mouse kidney tissue using Catenin beta Monoclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Human stomach cancer tissue using Catenin beta Monoclonal Antibody at dilution of 1:200.
客户发表文献
- 文献: Trans-Ancestry Mutation Landscape of Hepatoblastoma Genomes in Children
样品类型: HUH-6 cell,HepG2 cell
