CD146/MCAM Polyclonal Antibody
| 规格： | / 50μL / 100μL |
| 价格： | / ￥1010 / ￥1640 |
货号：D-AB-10421L
货期：咨询
宿主: Rabbit
反应性: H
应用: WB
Overview
| Synonyms | CD146,MCAM,MUC18 |
| Swissprot | P43121 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Recombinant Human CD146/MCAM protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304) |
| Recommended dilution | WB,,1:500-1:1000; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | CD146,also known as melanoma cell adhesion molecule (MCAM) or MUC18,originally identified as a biomarker of melanoma progression,is a transmembrane glycoprotein of 113-130 kDa,belonging to the immunoglobulin (Ig) superfamily. Structurally,it consists of five Ig domains,a transmembrane domain,and a cytoplasmic region. In normal adult tissue,CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis,and has been used as marker of circulating endothelium cells (CECs). In addition to the membrane-anchored form of CD146,a soluble form of CD146 (sCD146,105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells. This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F,which removes oligosaccharides from N-linked glycoproteins,led to a down-shift of the detected band. |
