CK19 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-40479
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | 40 kDa keratin intermediate filament, CK 19, CK-19, CK19, Cytokeratin 19, Cytokeratin-19, K19, K1C19, K1CS, Keratin 19, Keratin type I 40 kD, Keratin type I 40kD, Keratin type I cytoskeletal 19, Keratin, type I cytoskeletal 19, Keratin,type I, 40 kd, Keratin-19, KRT19,CK19抗体 |
| Swissprot | Q63279 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Rat Keratin, type I cytoskeletal 19 protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB 1:10000-1:30000 IHC 1:100-1:400 |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Intermediate filament, Keratin |
| Tissue specificity | Expressed in brain, heart, skin and in costameres of myoplasm at the sarcolemmal membrane in skeletal and cardiac muscle fibers. Undifferentiated gonads and somatic cells of ovarian cords throughout the fetal ovary development. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% Proclin300, 50% glycerol, pH7.3. |
| Background | The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19,one of type I keratins,is specifically expressed in the periderm,the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity,KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes,peripheral blood,and bone marrow of breast cancer patients. This antibody,generated against full length KRT19 protein,also recognizes KRT17,another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma. |
Images
Western Blot analysis of Caco-2 cells using CK19 Polyclonal Antibody at dilution of 1:20000.
Western Blot analysis of Rat intestine, Rat lung and Mouse lung using CK19 Polyclonal Antibody at dilution of 1:10000.
Immunohistochemistry of paraffin-embedded Human colon cancer using CK19 Polycloanl Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Human colon cancer using CK19 Polycloanl Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Rat kidney using CK19 Polycloanl Antibody at dilution of 1:200.
