Cleaved-PARP1 (D214) Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-30080
宿主: Rabbit
反应性: H,M
应用: WB,IHC-p,IF
Overview
| Synonyms | ADP-ribosyltransferase diphtheria toxin-like 1,ADPRT 1,ADPRT,ADPRT1,APOPAIN,ARTD1,NAD(+) ADP-ribosyltransferase 1,PARP,PARP-1,PARP1,PARP1,Poly [ADP-ribose] polymerase 1,Poly ADP ribose polymerase 1,Poly[ADP-ribose] synthase 1,PPOL,SCA1,Cleaved-PARP-1 (D214)抗体 |
| Swissprot | P09874 |
| Source | Rabbit |
| Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the Internal region of human PARP-1 |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-2000;IHC,,1:50-300;IF,,1:50-300; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Nucleus, nucleolus. Localizes at sites of DNA damage. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. |
Images
Western Blot analysis of A549 cells using Cleaved-PARP1 (D214) Polyclonal Antibody at dilution of 1:2000.
Immunohistochemistry of paraffin-embedded Human lung cancer tissue using Cleaved-PARP1 (D214) Polyclonal Antibody at dilution of 1:200.
Immunofluorescence analysis of Rat spleen tissue using Cleaved-PARP1 (D214) Polyclonal Antibody at dilution of 1:200.
客户发表文献
- 文献: Amentoflavone induces caspase-dependent/−independent apoptosis and dysregulates cyclin-dependent kinase-mediated cell cycle in colorectal cancer in vitro and in vivo
样品类型: HT-29 cell - 文献: Flavonoids from Rosa
roxburghii Tratt prevent reactive oxygen species-mediated DNA damage in thymus cells both combined with and without PARP-1 expression after exposure to radiation in vivo
样品类型: Tissue homogenate
