Cortisol Binding Globulin/CBG/SERPINA6 Polyclonal Antibody(Capture/Detector)
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN000440P
货期：咨询
宿主: Rabbit
反应性: H;M
应用: WB;IF;ELISA Capture/Detector
Overview
| Synonyms | Corticosteroid-binding gLobuLin;CBG;Serpin A6;Transcortin;SERPINA6;CBG |
| Swissprot | P08185 |
| Source | Rabbit |
| Reactivity | Human;Mouse |
| Immunogen | Recombinant Human Cortisol Binding Globulin/CBG/SERPINA6 SERPINA6 Protein expressed by Mammalian |
| Application | WB(Detection kit: E-IR-R304);IF;ELISA Capture/Detector |
| Recommended dilution | WB,,1:500-1:1000;IF,,1:50-1:200;ELISA Capture,,2-8 μg/mL;ELISA Detector,,0.1-0.4 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | Corticosteroid-binding globulin (CBG),also known as SerpinA6,is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG,and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context,CBG directs glucocorticoids to sites of inflammation,and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration,and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable,human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels. |
Images
Western Blot with Cortisol Binding Globulin/CBG/SERPINA6 Polyclonal Antibody at dilution of 1:500.Lane 1:Human plasma
Immunofluorescence analysis of NIH-3T3 cells using Cortisol Binding Globulin/CBG/SERPINA6 Polyclonal Antibody at dilution of 1:200
Sandwich ELISA-Recombinant Human Cortisol Binding Globulin/CBG/SERPINA6 SERPINA6 Protein standard curve.Background subtracted standard curve using Cortisol Binding Globulin/CBG/SERPINA6 antibody(AN000440P)(Capture),Cortisol Binding Globulin/CBG/SERPINA6 antibody(AN000440P)(Detector) in sandwich ELISA.The reference range value for Recombinant Human Cortisol Binding Globulin/CBG/SERPINA6 SERPINA6 Protein is 7.81-500ng/mL.
