Creatine Kinase (CK) Activity Assay Kit
肌酸激酶(CK)比色法测试盒
规格: | / 50 assays(48 samples) / 100 assays(96 samples) |
价格: | / ¥1600 / ¥2500 |
货号:E-BC-K558-S
检测仪器及波长: 紫外-可见光分光光度计(340 nm)
样本类型: 血清;血浆;动物组织;细胞
检测原理
磷酸肌酸激酶(CK,EC 2.7.3.2)催化磷酸肌酸和ADP生成肌酸和ATP,己糖激酶(HK,Hexokinase)催化肌酸与葡萄糖形成6-磷酸葡萄糖,6-磷酸葡萄糖脱氢酶(G6P-DH,Glucose-6-phosphate dehydrogenase)催化6-磷酸葡萄糖与NADP+生成NADPH,导致340 nm波长处吸光值增加。
关键信息
指标简称 | CK |
样本类型 | 血清;血浆;动物组织;细胞 |
灵敏度 | 3.7 U/L |
检测范围 | 3.7-160 U/L |
精密度 | 批间差:8.1%批内差:5.2% |
检测仪器及波长 | 分光光度计 |
保存条件 | 2-8℃ |
有效期 | 3个月 |
样本稀释倍数
在正式检测前,需选择的2-3例预期差异大的样本稀释成不同浓度进行预实验,结合本试剂盒的线性范围(3.7 U/L-160 U/L),选取最佳稀释倍数,进行正式批量实验。不同样本的稀释比例范围如下表(仅供参考):
样本 |
稀释倍数 |
样本 |
稀释倍数 |
人血清 |
不稀释 |
HepG2细胞匀浆 |
不稀释 |
人血浆 |
不稀释 |
10%大鼠肾匀浆 |
不稀释 |
小鼠血清 |
不稀释 |
10%大鼠脑匀浆 |
2-5 |
大鼠血清 |
不稀释 |
10%大鼠肝匀浆 |
2-10 |
注:稀释液为PBS(0.01 M,pH 7.4)。
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