Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)
肌酐(Cr)比色法测试盒(肌氨酸氧化酶法)
| 规格： | / 48T(32 samples) / 96T(80 samples) |
| 价格： | / ￥480 / ￥740 |
货号：E-BC-K188-M
检测仪器及波长: 酶标仪(510-520 nm，最佳检测波长515 nm)
样本类型: 血清;血浆;尿液
检测原理
肌酐在肌酐酶的催化下生成肌酸，肌酸在肌酸酶的催化下生成肌氨酸和尿素，肌氨酸再经肌氨酸氧化酶催化生成甘氨酸、甲醛和过氧化氢。过氧化氢与显色剂在过氧化物酶的催化下反应生成粉红色化合物，可通过测定515 nm波长下的OD值变化，来测定计算出肌酐的含量。
关键信息
| 指标简称 | Cr |
| 样本类型 | 血清;血浆;尿液 |
| 灵敏度 | 3.8 μmol/L |
| 检测范围 | 20.45-800 μmol/L |
| 精密度 | 批间差：3.7%批内差：1.4% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | 2-8℃ |
| 有效期 | 3个月 |
样本稀释倍数
在正式检测前，需选择预期差异较大的2-3个样本，稀释成不同浓度进行预实验，结合检测范围：38.2-800 μmol/L，不同样本稀释比例如下表(仅供参考)：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人尿液 |
20-30 |
大鼠血清 |
不稀释 |
|
人血清 |
不稀释 |
猪血清 |
不稀释 |
|
小鼠血清 |
参考低值样本检测 |
|
|
注：稀释液为生理盐水(0.9% NaCl)。在预实验中，样本绝对OD值低于0.005时，参考实验关键点中，低值样本的检测方法。
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