Elab Fluor® 647-conjugated Vinculin Monoclonal Antibody
| 规格： | / 25μL / 100μL |
| 价格： | / ￥350 / ￥920 |
货号：AN00297M
货期：咨询
宿主: Mouse
反应性: H
应用: FCM
Overview
| Synonyms | Vinculin;Metavinculin (MV);VCL |
| Swissprot | P18206 |
| Source | Mouse |
| Reactivity | Human |
| Immunogen | Recombinant human Vinculin protein expressed by E.coli |
| Application | FCM |
| Recommended dilution | FCM,,0.2 µg per 10^6 cells in a 100 µL suspension |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm>cytoskeleton. Cell junction>adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. |
| Clone No | Da-4D4 |
|---|---|
| Isotype | IgG1 |
| Purification | Protein A/G Purification |
| Conjugation | Elab Fluor®647 |
| Storage instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa,1,066-amino-acid protein which is ubiquitously expressed. Its splice variant,metavinculin (124 kDa),is muscle-specific. |
