EpCAM Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70132
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC,IF
Overview
| Synonyms | 17 1A,323/A3,Adenocarcinoma associated antigen,Adenocarcinoma-associated antigen,Antigen identified by monoclonal AUA1,AUA1,CD326,CD326 antigen,Cell surface glycoprotein Trop 1,Cell surface glycoprotein Trop 2,Cell surface glycoprotein Trop-1,CO 17A,CO17 1A,CO17A,DIAR5,EGP 2,EGP,EGP2,EGP314,EGP40,Ep CAM,Ep-CAM,EPCAM,EPCAM,EpCAM1,Epithelial cell adhesion molecule,Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD),Epithelial cell surface antigen,Epithelial cellular adhesion molecule,Epithelial glycoprotein 1,Epithelial glycoprotein 314,Epithelial glycoprotein,ESA,GA733 1,GA733 2,GA733-2,gastrointestinal tumor-associated antigen 2,35-KD glycoprotein,gp4,hEGP 2,hEGP314,HNPCC8,Human epithelial glycoprotein 2,KS 1/4 antigen,KS1/4,KSA,Ly74,Lymphocyte antigen 74,M1S 1,M1S2,M4S1,Major gastrointestinal tumor associated protein GA733 2,Major gastrointestinal tumor-associated protein GA733-2,mEGP314,Membrane component chromosome 4 surface marker (35kD glycoprotein),Membrane component,chromosome 4,surface marker 1,Membrane component,chromosome 4,surface marker,MIC18,MK 1,Protein 289A,TACD1,TACSTD1,TROP1,Tumor associated calcium signal transducer 1,Tumor associated calcium signal transducer 2 precursor,Tumor-associated calcium signal transducer 1,EpCAM抗体 |
| Swissprot | P16422,O55159,Q99JW5 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse EPCAM |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:1000;IHC,,1:300-1:800;IF,,1:200-1:500; |
| Concentration | 290 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm |
| Tissue specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.Highest expression level in jejunal mucosa. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Epithelial cell adhesion molecule (EpCAM,CD326) is a type I transmembrane glycoprotein that functions as a homophilic,epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion,EpCAM is also involved in cellular signaling,cell migration,proliferation,and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas,and has become a therapeutic target. |
Images
Western Blot analysis of various samples using EpCAM Polyclonal Antibody at dilution of 1:750.
Immunohistochemistry analysis of paraffin-embedded mouse colon using EpCAM Polyclonal Antibody at dilution of 1:300.
Immunofluorescence analysis of paraffin-embedded rat colon using EpCAM Polyclonal Antibody at dilution of 1:300.
