Flag-Tag Monoclonal Antibody
| 规格： | / 30μL / 60μL / 120μL / 300μL / 500μL |
| 价格： | / ￥295 / ￥548 / ￥875 / ￥1975 / ￥2835 |
货号：E-AB-20006
宿主: Mouse
应用: WB,IF,IP
Overview
| Synonyms | DDDDK epitope tag,DDDDK epitope tag,DYKDDDDK epitope tag,Flag-Tag抗体 |
| Source | Mouse |
| Immunogen | Synthetic Peptide |
| Application | WB(Detection kit: E-IR-R304),IF,IP |
| Recommended dilution | WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Clone No | Clone:4A2 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.4 |
| Background | Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. |
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