Galectin-3 Polyclonal Antibody
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN000300P
货期：咨询
宿主: Rabbit
反应性: H;M
应用: IHC;IF;ELISA Capture/Detector
Overview
| Synonyms | GAL3;GaL-3;GaLectin-3;35 kDa Lectin;Carbohydrate-binding protein 35 (CBP 35);GaLactose-specific Lectin 3;GaLactoside-binding protein (GALBP);IgE-binding protein;LGALS3;MAC2 |
| Swissprot | P17931 |
| Source | Rabbit |
| Reactivity | Human;Mouse |
| Immunogen | Recombinant Human Galectin-3 protein expressed by E.coli |
| Application | IHC(Detection kit: E-IR-R213);IF;ELISA Capture/Detector |
| Recommended dilution | IHC,,1:650-1:1250;IF,,1:50-1:200;ELISA Capture,,2-8 μg/mL;ELISA Detector,,0.1-0.4 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | Galactose-specific lectin which binds IgE. May mediate with the alpha-3,beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1,required for terminal differentiation of columnar epithelial cells during early embryogenesis.In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion,chemoattraction of monocytes macrophages,opsonization of apoptotic neutrophils,and activation of mast cells. Together with TRIM16,coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16l1 and BECN1 in response to damaged endomembranes. |
Images
Immunohistochemistry of paraffin-embedded Human colon cancer using Galectin-3 Polyclonal Antibody at dilution of 1:1250
Immunofluorescence analysis of NIH-3T3 cells using Galectin-3 Polyclonal Antibody at dilution of 1:200
Sandwich ELISA-Recombinant Human Galectin-3 protein standard curve.Background subtracted standard curve using Galectin-3 antibody(AN000300P)(Capture),Galectin-3 antibody(AN000300P)(Detector) in sandwich ELISA.The reference range value for Recombinant Human Galectin-3 protein is 0.15625-10 ng/mL.
