GAPDH Polyclonal Antibody
| 规格： | / 30μL / 60μL / 120μL / 300μL / 500μL |
| 价格： | / ￥295 / ￥548 / ￥875 / ￥1975 / ￥2835 |
货号：E-AB-40337
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | aging associated gene 9 protein, BARS-38, cb609, G3PDH, GAPD, KNC-NDS6, OCAS, p38 component,GAPDH抗体 |
| Swissprot | Q5XJ10 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant Zebrafish Glyceraldehyde-3-phosphate dehydrogenase protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:3000-1:6000;IHC,,1:300-1:500; |
| Concentration | 0.66mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm, Cytoskeleton, Nucleus |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus post modification such as S-nitrosylation. |
Images
Western Blot analysis of A431 and Hela cells using GAPDH Polyclonal Antibody at dilution of 1:5000
Western Blot analysis of HepG2, HT-29, A549, Jurkat, MCF-7, Rat heart, Mouse brain, Mouse heart and Rat brain using GAPDH Polyclonal Antibody at dilution of 1:5000
Immunohistochemistry of paraffin-embedded Human bladder cancer using GAPDH Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human kidney using GAPDH Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Mouse colon using GAPDH Polyclonal Antibody at dilution of 1:400
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