HA-Tag Monoclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-48021
宿主: Mouse
反应性: All
应用: WB, IF,IP,FCM
Overview
| Synonyms | HA epitope tag, HA1 ,HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain |
| Source | Mouse |
| Reactivity | All |
| Immunogen | Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. |
| Application | WB(Detection kit: E-IR-R304), IF,IP,FCM |
| Recommended dilution | WB,,1:5000-10000;IP,,3ug/sample;IF,,1:400-800;FCM,,0.2ug; |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Isotype | IgG2a |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze/ thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. |
Images
Western blotting with anti-HA-Tag monoclonal antibody at dilution of 1:5000.Lane 1: HA tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with HA-Tag fusion protein using anti-HA-Tag monoclonal antibody at dilution of 1:800.
IP Result of 293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag monoclonal antibody .lane 1: mouse IgG2a Isotype Control, Lane 2:input, Lane 3: anti-HA-Tag mouse monoclonal antibody
1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag mAb(E-AB-48021) and AF647 conjugated Goat Anti-mouse IgG (H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
