HA-Tag Polyclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-40523
宿主: Rabbit
反应性: All
应用: WB, IF,IP,FCM
Overview
| Synonyms | HA epitope tag, HA1, HA2, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain |
| Source | Rabbit |
| Reactivity | All |
| Immunogen | Synthetic peptide corresponding to HA tag conjugated to keyhole limpet haemocyanin. |
| Application | WB(Detection kit: E-IR-R304), IF,IP,FCM |
| Recommended dilution | WB,,1:2000-5000;IP,,3ug/sample;IF,,1:800-1600;FCM,,0.2ug; |
| Concentration | 4mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. |
Images
Western blotting with Anti-HA rabbit polyclonal antibody at dilution of 1:1000. Lane1: HA tag transfected HEK 293 whole cell lysate, Lane2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with the HA-GFP, using anti-HA-Tag Polyclonal Antibody at 1:800 dilution.
IP Result of 293F cells transfected with HA-Tag fusion protein,using anti-HA-Tag rabbit polyclonal antibody. lane 1: rabbit IgG Isotype Control, Lane 2:input, Lane 3: anti-HA-Tag rabbit antibody
1x10^6 CHO cells Transfected with a HA plasmid were stained with 0.2ug Anti-HA tag pAb(E-AB-40523) and AF647 conjugated Goat Anti-Rabbit IgG (H+L);lsotype Control stained with 0.2ug Rabbit IgG and AF647 conjugated Goat Anti-Rabbit IgG (H+L)
