HLA-DOB Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-18081
宿主: Rabbit
反应性: H
应用: WB,IHC
Overview
| Synonyms | HLA-DOB,HLA class II histocompatibility antigen,DO beta chain,MHC class II antigen DOB,HLA-DOB抗体 |
| Swissprot | P13765 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | Synthetic peptide of human HLA-DOB |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:30-1:150; |
| Concentration | 1.2mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Endosome membrane,Lysosome,lysosomal membrane,lysosome,Plasma Membrane,MHC class II protein complex,Other locations:integral component of membrane |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
| Background | HLA-DOB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane. It is located in intracellular vesicles. DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. HLA-DOB (Major Histocompatibility Complex, Class II, DO Beta) is a Protein Coding gene. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and CTLA4 Signaling. GO annotations related to this gene include MHC class II protein complex binding and MHC class II receptor activity. An important paralog of this gene is HLA-DRB1. |
Images
Western blot analysis of 293T cell lysate using HLA-DOB Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human tonsil tissue using HLA-DOB Polyclonal Antibody at dilution of 1:40(×200)
Immunohistochemistry of paraffin-embedded Human prost ate cancer tissue using HLA-DOB Polyclonal Antibody at dilution of 1:40(×200)
