HMGB-1 Polyclonal Antibody(Capture/Detector)
| 规格： | / 25μg / 100μg |
| 价格： | / ￥800 / ￥2100 |
货号：AN000120P
货期：咨询
宿主: Rabbit
反应性: H;M;R
应用: WB;ELISA Capture/Detector
Overview
| Synonyms | HMGB1;HMG1;High mobiLity group protein B1;High mobiLity group protein 1 (HMG-1) |
| Swissprot | P09429 |
| Source | Rabbit |
| Reactivity | Human;Mouse;Rat |
| Immunogen | Recombinant Human HMGB-1 protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304);ELISA Capture/Detector |
| Recommended dilution | WB,,1:500-1:1000;ELISA Capture,,2-8 μg/mL;ELISA Detector,,0.1-0.4 μg/mL |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Isotype | IgG |
| Purification | Antigen Affinity Purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at 4°C Valid for 1 month or -20°C Valid for 12 months,Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions. |
| Storage buffer | Sterile PBS,pH 7.4 |
| Background | Human High-mobility group box 1 protein (HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly,both as an inflammatory mediator that promotes monocyte migration and cytokine secretion,and as a mediator of T cell-dendritic cell interaction. The cytokine activity of HBMG1 is restricted to the HMG B box,while the A box is associated with the helix-loop-helix domain of transcription factors. HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence,it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted,HMGB1 transduces cellular signals through its high affinity receptor,RAGE and,possibly,TlR2 and TlR4. Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse,rat,bovine and porcine HMGB1,respectively. |
Images
Western Blot with HMGB-1 Polyclonal Antibody at dilution of 1:1000.Lane 1:Hela cell lysate,Lane 2:Jurkat cell lysate,Lane 3:A431 cell lysate,Lane 4:NIH/3T3 cell lysate,Lane 5:C6 cell lysate.
Sandwich ELISA-Recombinant Human HMGB-1 protein standard curve.Background subtracted standard curve using HMGB-1 antibody(AN000120P)(Capture),HMGB-1 antibody(AN000120P)(Detector) in sandwich ELISA.The reference range value for Recombinant Human HMGB-1 protein is 31.25-2000 pg/mL.
