HRP-conjugated GAPDH Monoclonal Antibody
| 规格： | / 25μL / 100μL |
| 价格： | / ￥280 / ￥1050 |
货号：D-AB-20141L
货期：咨询
宿主: Mouse
反应性: H,M
应用: WB
Overview
| Synonyms | ACTB;Actin beta;Actx;Actin cytoplasmic 1;b actin;A26C1A |
| Swissprot | Q7ZVF9 |
| Source | Mouse |
| Reactivity | Human, Mouse |
| Immunogen | Recombinant human GAPDH protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304) |
| Recommended dilution | WB,,1:2500-1:5000 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm>cytosol. Nucleus. Cytoplasm>perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1,which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions. |
| Clone No | Da-8D6 |
|---|---|
| Isotype | IgG2a |
| Purification | Protein A/G Purification |
| Conjugation | HRP |
| Storage instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Storage buffer | PBS with 0.1% procline 300,1% BSA and 50% glycerol,pH7.4 |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |
