Human IL-1β(Interleukin 1 Beta) ELISA Kit
人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2400 / ￥1650 / ￥10200 |
货号：E-EL-H0149
反应性: 人
检测范围: 7.81~500 pg/mL
灵敏度: 4.69 pg/mL
用途
该试剂盒用于体外定量检测人serum, plasma and other biological fluids中IL-1β浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品或标准品中的人IL-1β会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素。抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。
| 反应类型 | 夹心法 |
| 规格 | 96T |
| 反应时间 | 3.5h |
| 反应性 | 人 |
| 检测方法 | Colormetric |
| 检测范围 | 7.81—500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100μL |
| 样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的人IL-1β，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 500 | 2.387 2.403 |
2.395 | 2.318 |
| 250 | 1.684 1.706 |
1.695 | 1.618 |
| 125 | 0.956 0.932 |
0.944 | 0.867 |
| 62.5 | 0.532 0.538 |
0.535 | 0.458 |
| 31.25 | 0.299 0.279 |
0.289 | 0.212 |
| 15.63 | 0.192 0.168 |
0.18 | 0.103 |
| 7.81 | 0.123 0.137 |
0.13 | 0.053 |
| 0 | 0.068 0.085 |
0.077 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) |
27.70 | 46.20 | 197.60 | 29.10 | 47.80 | 192.60 |
| Standard
deviation |
1.40 | 2.60 | 9.50 | 1.70 | 2.80 | 6.00 |
| C V (%) | 5.05 | 5.63 | 4.81 | 5.84 | 5.86 | 3.12 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 94-110 | 100 |
| EDTA plasma (n=5) | 89-102 | 95 |
| Cell culture media (n=5) | 84-98 | 91 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 95-109 | 88-100 | 94-108 |
| Average (%) | 102 | 94 | 102 | |
| 1:4 | Range (%) | 95-109 | 82-94 | 93-105 |
| Average (%) | 100 | 88 | 99 | |
| 1:8 | Range (%) | 98-110 | 82-95 | 97-109 |
| Average (%) | 105 | 88 | 103 | |
| 1:16 | Range (%) | 96-108 | 80-91 | 97-113 |
| Average (%) | 102 | 86 | 103 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃，可存放6个月 |
| 冻干标准品 | Reference Standard | 2 支 | |
| 浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
| 浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光)，可存放6个月 |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
| 生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱，双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
|
1.对应板孔中加入100μL标准品工作液或样本，37℃孵育90分钟 |
| 2.弃掉板内液体后，立即加入100μL生物素化抗体工作液，37℃孵育60分钟 | |
| 3.弃掉板内液体，洗板3次 | |
| 4.每孔加入100μL HRP酶结合物工作液，37℃孵育30分钟，弃掉板内液体，洗板5次 | |
| 5.每孔加入90μL底物溶液，37℃孵育15分钟 | |
| 6.每孔加入50μL终止液 | |
| 7.立即在450nm波长下读数，处理数据 |
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样品类型: Serum - 文献: LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203
样品类型: Cell culture supernatant - 文献: N6-methyladenosine reader YTH N6-methyladenosine RNA binding protein 3 or insulin like growth factor 2 mRNA binding protein 2 knockdown protects human bronchial epithelial cells from hypoxia/reoxygenation injury by inactivating p38 MAPK, AKT, ERK1/2, and
样品类型: BEAS-2B cell - 文献: Knock-down of microRNA miR-556-5p increases cisplatin-sensitivity in non-small cell lung cancer (NSCLC) via activating NLR family pyrin domain containing 3 (NLRP3)-mediated pyroptotic cell death
样品类型: A549 cell,H1299 cell - 文献: Dihydroartemisinin targets fibroblast growth factor receptor 1 (FGFR1) to inhibit interleukin 17A (IL-17A)-induced hyperproliferation and inflammation of keratinocytes
样品类型: HaCaT cell - 文献: Circ_0001897 regulates high glucose-induced angiogenesis and inflammation in retinal microvascular endothelial cells through miR-29c-3p/transforming growth factor beta 2 axis
样品类型: Human retinal microvascular endothelial cell (HRECs) - 文献: Carvedilol activates nuclear factor E2-related factor 2/ antioxidant response element pathway to inhibit oxidative stress and apoptosis of retinal pigment epithelial cells induced by high glucose
样品类型: ARPE-19 cell - 文献: DosR antigen Rv1737c induces activation of macrophages dependent on the TLR2 pathway
样品类型: THP-1 cells - 文献: The first cloned echinoderm tumor necrosis factor receptor from Holothuria leucospilota: Molecular characterization and functional analysis
样品类型: Cell culture supernatant - 文献: Cancer cell-derived exosomal LINC00313 induces M2 macrophage differentiation in non-small cell lung cancer
样品类型: THP-1 cell,H1299 cell - 文献: iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin–angiotensin system-mediated congenital ureteropelvic junction obstruction
样品类型: Serum,Cell sample - 文献: Long non-coding RNA NEAT1 promotes lipopolysaccharide-induced injury in human tubule epithelial cells by regulating miR-93-5p/TXNIP axis
样品类型: Cell culture supernatant - 文献: Cul4a attenuates LPS-induced acute kidney injury via blocking NF-kB signaling pathway in sepsis
样品类型: HK-2 cell - 文献: CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis
样品类型: Serum - 文献: Phillygenin inhibits LPS-induced activation and inflammation of LX2 cells by TLR4/MyD88/NF-κB signaling pathway
样品类型: Cell culture supernatant - 文献: Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology
样品类型: Cell culture supernatant - 文献: Hyperoxia Induced Bronchopulmonary Dysplasia-Like Inflammation via miR34a-TNIP2-IL-1β Pathway
样品类型: sputum - 文献: Role of aldosterone in the activation of primary mice hepatic stellate cell and liver fibrosis via NLRP3 inflammasome
样品类型: Serum,Tissue homogenate - 文献: Eriodictyol inhibits survival and inflammatory responses and promotes apoptosis in rheumatoid arthritis fibroblast‐like synoviocytes through AKT/FOXO1 signaling
样品类型: rheumatoid arthritis (RA) fibroblast-like synoviocytes - 文献: Wedelolactone ameliorates Pseudomonas aeruginosa-induced inflammation and corneal injury by suppressing caspase-4/5/11/GSDMD-mediated non-canonical pyroptosis
样品类型: piHCKs - 文献: Association between pro-inflammatory cytokines in cerebrospinal fluid and headache in patients with aneurysmal subarachnoid hemorrhage
样品类型: cerebrospinal fluid (CSF) - 文献: Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis
样品类型: serum - 文献: Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1
样品类型: SH-SY5Y cell - 文献: Evaluation of IL-23p19/Ebi3 (IL-39) gingival crevicular fluid levels in periodontal health, gingivitis, and periodontitis
样品类型: gingival crevicular fluid (GCF) - 文献: Proanthocyanidins attenuate the high glucose-induced damage of retinal pigment epithelial cells by attenuating oxidative stress and inhibiting activation of the NLRP3 inflammasome
样品类型: ARPE-19 cell - 文献: Myrtenal and β-caryophyllene oxide screened from Liquidambaris Fructus suppress NLRP3 inflammasome components in rheumatoid arthritis
样品类型: serum,RA-FLS - 文献: strain FP10
样品类型: Cell culture supernatant - 文献: Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
样品类型: Serum,Cell culture supernatant - 文献: Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
样品类型: Wound drainage fluid - 文献: Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein
样品类型: Serum - 文献: Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production
样品类型: Serum,Plasma,Cell sample - 文献: Construction of an exposure-pathway-phenotype in children with depression due to transfusion-dependent thalassemia: Results of (un)supervised machine learning.
样品类型: Serum - 文献: Effect and mechanism of phospholipid scramblase 4 (PLSCR4) on lipopolysaccharide (LPS)-induced injury to human pulmonary microvascular endothelial cells
样品类型: HPMECs - 文献: Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF
样品类型: adipose-derived stem cell (ASCs) - 文献: Mammalian Ste20-like kinase 4 inhibits the inflammatory response in Aspergillus fumigatus keratitis
样品类型: Cell lysate - 文献: Inhibition of HMGB1 involved in the protective of salidroside on liver injury in diabetes mice
样品类型: Cell culture supernatant - 文献: IL-38 alleviates the inflammatory response and the degeneration of nucleus pulposus cells via inhibition of the NF-κB signaling pathway in vitro
样品类型: Cell culture supernatant - 文献: Asymmetric and symmetric dimethylarginine gingival crevicular fluid levels in periodontitis
样品类型: Gingival crevicular fluid(GCF) - 文献: Silencing of UCA1 Protects Against MPP+-Induced Cytotoxicity in SK-N-SH Cells via Modulating KCTD20 Expression by Sponging miR-423-5p
样品类型: Cell culture supernatant - 文献: Validation of Salivary Markers, IL-1β, IL-8 and Lgals3bp for Detection of Oral Squamous Cell Carcinoma in an Indian Population
样品类型: Saliva - 文献: Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits
样品类型: Cell culture supernatant - 文献: Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users:
样品类型: Plasma - 文献: Penehyclidine hydrochloride alleviates lipopolysaccharide‑induced acute respiratory distress syndrome in cells via regulating autophagy‑related pathway
样品类型: Cell culture supernatant - 文献: Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial
样品类型: blood - 文献: NF-κB/ABCA1 pathway aggravates ox-LDL-induced cell pyroptosis by activation of NLRP3 inflammasomes in THP-1-derived macrophages
样品类型: THP-1 monocytes - 文献: Exploring potential serum levels of Homocysteine, interleukin-1 beta, and apolipoprotein B 48 as new biomarkers for patients with ischemic stroke
样品类型: serum - 文献: A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions
样品类型: Serum - 文献: Total Flavonoids of Engelhardia roxburghiana Wall. Leaves Alleviated Foam Cells Formation through AKT/mTOR-Mediated Autophagy in the Progression of Atherosclerosis
样品类型: THP-1 cell - 文献: Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line
样品类型: Cell lysate - 文献: MicroRNA‑16 regulates lipopolysaccharide‑induced inflammatory factor expression by targeting TLR4 in normal human bronchial epithelial cells
样品类型: NHBE cells - 文献: Mangiferin inhibits hypoxia/reoxygenation‑induced alveolar epithelial cell injury via the SIRT1/AMPK signaling pathway
样品类型: A549 cell - 文献: MiR-223-3p and miR-22-3p inhibit monosodium urate-induced gouty inflammation by targeting NLRP3
样品类型: Cell culture supernatant(THP-1) - 文献: LncRNA PMS2L2 downregulates miR-24 through methylation to suppress cell apoptosis in ulcerative colitis
样品类型: HCnEpCs - 文献: Exosomes derived from human umbilical cord mesenchymal stem cells alleviate acute liver failure by reducing the activity of the NLRP3 inflammasome in macrophages
样品类型: Serum - 文献: The Combination of Insulin Resistance and Serum Interleukin-1β Correlates with Post-Stroke Depression in Patients with Acute Ischemic Stroke
样品类型: Serum - 文献: XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485
样品类型: Serum - 文献: Upregulated NORAD is implicated in apoptosis, inflammation, and oxidative stress in ulcerative colitis through the nuclear factor-κappaB signaling
样品类型: human normal colonic mucosal cells (FHCs) - 文献: The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF-κB Signaling Pathway
样品类型: THP-1 cells - 文献: Antiplatelet Activity of Tetramethylpyrazine via Regulation of the P2Y12 Receptor Downstream Signaling Pathway
样品类型: platelets suspensions - 文献: Downregulation of hsa_circ_0068087 ameliorates TLR4/NF-κB/NLRP3 inflammasome-mediated inflammation and endothelial cell dysfunction in high glucose conditioned by sponging miR-197
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Inhibition of microRNA-665 Alleviates Septic Acute Kidney Injury by Targeting Bcl-2
样品类型: HK-2 cell - 文献: Antidiabetic (type 2) effects of Lactobacillus G15 and Q14 in rats through regulation of intestinal permeability and microbiota
样品类型: Plasma,Serum - 文献: Granulocyte-macrophage colony-stimulating factor (GM-CSF) in subjects with different stages of periodontitis according to the new classification
样品类型: gingival crevicular fluid (GCF) - 文献: Long non-coding RNA MEG3 regulates CSE-induced apoptosis and inflammation via regulating miR-218 in 16HBE cells
样品类型: Cell culture supernatant - 文献: The effect of conventional versus electronic cigarette use on treatment outcomes of peri-implant disease
样品类型: Saliva - 文献: Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1–7) upregulation
样品类型: Cell culture supernatant,Serum - 文献: Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model
样品类型: Ker-CT cell,HaCaT cell - 文献: Ceramide induces pyroptosis through TXNIP/NLRP3/GSDMD pathway in HUVECs
样品类型: Human Umbilical Vein Endothelial Cells (HUVECs) - 文献: Periodontal Ligament Stem Cells Reverse the High Glucose Level-Induced Inflammatory State of Macrophages
样品类型: THP-1 cell - 文献: Exploration of the mechanism by which Huangqi Guizhi Wuwu decoction inhibits Lps-induced inflammation by regulating macrophage polarization based on network pharmacology
样品类型: human histiocytic lymphoma (U-937) cell - 文献: Effectiveness of a Cucumber Extract Supplement on Articular Pain in Patients with Knee Osteoarthritis: A Randomized Double-Blind Controlled Clinical Trial
样品类型: plasma - 文献: XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway
样品类型: HRGECs - 文献: Carotid intima-media thickness and serum proinflammatory cytokine levels in rosacea patients without cardiovascular risk factors
样品类型: Serum - 文献: Exogenous hydrogen sulfide mitigates LPS + ATP-induced inflammation by inhibiting NLRP3 inflammasome activation and promoting autophagy in L02 cells
样品类型: Cell culture supernatant - 文献: Tacrolimus Inhibits TNF-α/IL-17A-Produced pro-Inflammatory Effect on Human Keratinocytes by Regulating IκBζ
样品类型: Cell culture supernatant - 文献: The Neuroimmune and Neurotoxic Fingerprint of Major Neurocognitive Psychosis or Deficit Schizophrenia: a Supervised Machine Learning Study
样品类型: serum - 文献: Association of PAR-2 Gene Polymorphisms with the Inflammatory Response and Susceptibility to Knee Osteoarthritis in the Chinese Han Population
样品类型: Synovia - 文献: Inhibition of miR‑27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells
样品类型: Cell culture supernatant - 文献: HMGB‐1, TLR4, IL‐1R1, TNF‐α, and IL‐1β: novel epilepsy markers?
样品类型: serum - 文献: MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2
样品类型: Beas2B cells - 文献: Role of miR‑589‑3p in human lumbar disc degeneration and its potential mechanism
样品类型: Cell culture supernatant - 文献: N‑methyl pyrrolidone promotes ankle fracture healing by inhibiting inflammation via suppression of the mitogen‑activated protein kinase signaling pathway
样品类型: peripheral blood - 文献: Anti‑inflammatory effect of Resolvin D1 on LPS‑treated MG‑63 cells Corrigendum in /10.3892/etm.2019.8021
样品类型: Serum - 文献: Sex difference in IL-6 modulation of cognition among Chinese individuals with major depressive disorder
样品类型: Serum - 文献: Doxycycline inhibits NAcht Leucine-rich repeat Protein 3 inflammasome activation and interleukin-1β production induced by Porphyromonas gingivalis-lipopolysaccharide and adenosine triphosphate in human gingival fibroblasts
样品类型: Cell culture supernatant - 文献: Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in vitro by inhibiting oxidative stress and inflammatory responses
样品类型: Cell culture supernatant - 文献: Effects of quercetin on diabetic retinopathy and its association with NLRP3 inflammasome and autophagy
样品类型: Cell culture supernatant - 文献: Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-κB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children
样品类型: Serum - 文献: Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response
样品类型: Serum - 文献: Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments
样品类型: Serum - 文献: Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells
样品类型: Cell culture supernatant - 文献: Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1
样品类型: Cell culture supernatant - 文献: Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via the AMPK-mTOR pathway
样品类型: L02 cells - 文献: In Vitro Immunomodulatory Effect of Food Supplement from Aloe vera
样品类型: Cell culture supernatant - 文献: Comparing the ICSI outcome between different causes of subfertility and estimate the role of IL-1β in predicting ICSI outcome.
样品类型: follicular fluid,serum - 文献: Dysfunctional oxidative stress response in first-episode of schizophrenia
样品类型: serum
