Human INS(Insulin) ELISA Kit
人胰岛素(INS)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2400 / ￥1650 / ￥10200 |
货号：E-EL-H2665
反应性: 人
检测范围: 0.78~50 μIU/mL
灵敏度: 0.47 μIU/mL
用途
该试剂盒用于体外定量检测人serum, plasma and other biological fluids中INS浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人INS抗体包被于酶标板上，实验时样品或标准品中的人INS会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人INS抗体和辣根过氧化物酶标记的亲和素。抗人INS抗体与结合在包被抗体上的人INS结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。
| 反应类型 | 夹心法 |
| 规格 | 96T |
| 反应时间 | 3.5h |
| 反应性 | 人 |
| 检测方法 | Colormetric |
| 检测范围 | 0.78—50 μIU/mL |
| 灵敏度 | 0.47 μIU/mL |
| 样本体积 | 100μL |
| 样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的人INS，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (μIU/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 50 | 2.491 2.572 |
2.532 | 2.476 |
| 25 | 1.671 1.672 |
1.672 | 1.616 |
| 12.5 | 0.933 0.936 |
0.935 | 0.879 |
| 6.25 | 0.508 0.457 |
0.483 | 0.427 |
| 3.13 | 0.265 0.269 |
0.267 | 0.211 |
| 1.57 | 0.184 0.178 |
0.181 | 0.125 |
| 0.78 | 0.112 0.117 |
0.115 | 0.059 |
| 0 | 0.055 0.057 |
0.056 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (μIU/mL) |
2.38 | 5.03 | 23.63 | 2.21 | 6.19 | 21.47 |
| Standard
deviation |
0.13 | 0.25 | 1.25 | 0.11 | 0.29 | 0.97 |
| C V (%) | 5.46 | 4.97 | 5.29 | 4.98 | 4.68 | 4.52 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 88-99 | 94 |
| EDTA plasma (n=5) | 96-108 | 101 |
| Cell culture media (n=5) | 94-106 | 102 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 87-95 | 98-110 | 96-108 |
| Average (%) | 90 | 104 | 102 | |
| 1:4 | Range (%) | 95-105 | 88-98 | 86-95 |
| Average (%) | 99 | 95 | 92 | |
| 1:8 | Range (%) | 98-108 | 93-103 | 98-111 |
| Average (%) | 105 | 100 | 104 | |
| 1:16 | Range (%) | 93-106 | 82-95 | 100-112 |
| Average (%) | 99 | 90 | 104 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃，可存放6个月 |
| 冻干标准品 | Reference Standard | 2 支 | |
| 浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
| 浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光)，可存放6个月 |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
| 生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱，双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
|
1.对应板孔中加入100μL标准品工作液或样本，37℃孵育90分钟 |
| 2.弃掉板内液体后，立即加入100μL生物素化抗体工作液，37℃孵育60分钟 | |
| 3.弃掉板内液体，洗板3次 | |
| 4.每孔加入100μL HRP酶结合物工作液，37℃孵育30分钟，弃掉板内液体，洗板5次 | |
| 5.每孔加入90μL底物溶液，37℃孵育15分钟 | |
| 6.每孔加入50μL终止液 | |
| 7.立即在450nm波长下读数，处理数据 |
客户发表文献
- 文献: Glucose-responsive microneedle patch for closed-loop dual-hormone delivery in mice and pigs
样品类型: plasma - 文献: Branched Chain Amino Acids, New Target of Germinated Brown Rice against Type 2 Diabetes Mellitus: A Randomized Controlled Trial
样品类型: serum - 文献: Prevalence of ApoB100 rs693 gene polymorphism in metabolic syndrome among female students at King Abdulaziz University
样品类型: Serum - 文献: Vitamin E Levels in Ethnic Communities in Malaysia and Its Relation to Glucose Tolerance, Insulin Resistance and Advanced Glycation End Products: A Cross-Sectional Study
样品类型: serum - 文献: Intermittent Fasting Enhanced the Cognitive Function in Older Adults with Mild Cognitive Impairment by Inducing Biochemical and Metabolic changes: A 3-Year Progressive Study
样品类型: Plasma - 文献: Cardiovascular Disease Prediction by Machine Learning Algorithms Based on Cytokines in Kazakhs of China
样品类型: blood - 文献: Metabolomics and correlation network analyses of core biomarkers in type 2 diabetes
样品类型: Plasma - 文献: Ramadan diurnal intermittent fasting modulates SOD2, TFAM, Nrf2, and sirtuins (SIRT1, SIRT3) gene expressions in subjects with overweight and obesity
样品类型: serum - 文献: The effect of hydrothermal processing on the performance of broiler chicks fed corn or sorghum-based diets
样品类型: Serum - 文献: The protective effect of N-acetyl cysteine against selenium toxicity and gamma irradiation in rats
样品类型: blood - 文献: Nomogram Based on Cytokines for Cardiovascular Diseases in Xinjiang Kazakhs
样品类型: plasma - 文献: Comparing acute effects of extra virgin coconut oil and extra virgin olive oil consumption on appetite and food intake in normal-weight and obese male subjects
样品类型: serum - 文献: Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study
样品类型: serum - 文献: Assessment of Serum Chemerin Levels in Acanthosis Nigricans: A Case Control Study
样品类型: serum - 文献: Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial
样品类型: plasma - 文献: Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity
样品类型: plasma - 文献: Serum profile of cytokines and their genetic variants in metabolic syndrome and healthy subjects: a comparative study
样品类型: serum - 文献: Insulin Resistance is Associated with Gut Permeability Without the Direct Influence of Obesity in Young Adults
样品类型: Plasma - 文献: Ramadan Intermittent Fasting Is Associated with Changes in Circulating Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in Metabolically Healthy Obese Subjects
样品类型: serum - 文献: The role of biofield energy treatment on psychological symptoms, mental health disorders, and stress-related quality of life in adult subjects: A randomized controlled clinical trial
样品类型: serum - 文献: Impacts of a nutrition education intervention in individuals with cardiometabolic risk: Protocol of a randomized controlled trial
样品类型: plasma - 文献: Effect of saccharin, a non-nutritive sweeteners, on insulin and blood glucose levels in healthy young men: A crossover trial
样品类型: plasma - 文献: Association of Apelin gene rs3115757 variation and expression with metabolic syndrome among female students in King Abdulaziz University
样品类型: serum - 文献: Peroxisome proliferator activated receptor gamma 34C>G variant and anthropometric parameters in metabolic syndrome
样品类型: serum - 文献: Effect of Preoperative Oral Carbohydrate Administration on Patients Undergoing Cesarean Section with Epidural Anesthesia: A Pilot Study
样品类型: serum
