Lactate Dehydrogenase (LDH) Activity Assay Kit (WST-8 Method)
乳酸脱氢酶(LDH)比色法测试盒(WST-8法)
| 规格： | / 48T(32 samples) / 96T(80 samples) |
| 价格： | / ￥500 / ￥880 |
货号：E-BC-K766-M
检测仪器及波长: 酶标仪(450 nm)
样本类型: 动物组织样本;血清(浆);胸水及细胞样本
检测原理
乳酸脱氢酶催化乳酸和NAD+反应产生丙酮酸和NADH，NADH在PMS作用下，将电子传递给WST-8，生产黄色的产物，其在450 nm有特征吸收峰。
关键信息
| 指标简称 | LDH |
| 样本类型 | 动物组织样本;血清(浆);胸水及细胞样本 |
| 灵敏度 | 0.11 U/L |
| 检测范围 | 0.11-39.9 U/L |
| 精密度 | 批间差：2.3%批内差：2.3% |
| 检测仪器及波长 | 酶标仪 |
| 保存条件 | -20℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，根据预实验的结果，结合本试剂盒的线性范围(0.11-39.9 U/L)，请参考下表稀释(仅供参考)：
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
10-20 |
10%大鼠脾组织 |
150-250 |
|
狗血清 |
10-20 |
10%小鼠肝组织 |
250-350 |
|
小鼠血清 |
50-100 |
10%大鼠肾组织 |
250-350 |
|
食蟹猴血清 |
10-20 |
10%大鼠肺组织 |
250-350 |
注：稀释液为试剂一。
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