MBP Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70265
宿主: Rabbit
反应性: M,R
应用: WB,IHC
Overview
| Synonyms | GDB,Golli MBP,Golli MBP,myelin basic protein,Hemopoietic MBP,HMBPR,HUGO,MBP,MBP_CAVPO,MBP,MGC99675,MLD,Myelin A1 protein,Myelin A1 Protein,basic,Myelin basic protein,Myelin Deficient,Myelin membrane encephalitogenic protein,OTTHUMP00000163776,OTTHUMP00000174387,OTTHUMP00000174388,SHI,Shiverer,SP,MBP抗体 |
| Swissprot | P04370,P02688 |
| Source | Rabbit |
| Reactivity | Mouse,Rat |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Myelin Basic Protein |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:500-1:1000;IHC,,1:200-1:600; |
| Concentration | 300 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Myelin membrane. Cytoplasmic side of myelin. |
| Tissue specificity | In the embryo, isoform 1-isoform 3 are found in neurons within the central nervous system (primarily in pioneer neurons important in the formation of the cortex) and the peripheral nervous system. They are also expressed in the thymus, gut, lung and kidney. In the adult, isoform 1-isoform 3 are highly expressed in the brain (mainly in brain regions rich in oligodendrocytes) and spleen. Lower levels are seen in the heart, kidney and lung. Isoform 2 is also found in cells of the immune system. The isoforms missing the 134 first amino acids (isoform 4-isoform 13) are almost exclusively produced in the myelin-forming cells, the mature oligodendrocytes.Highest expression level in sciatic nerve. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP. |
Images
Western Blot analysis of various samples using MBP Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry analysis of paraffin-embedded mouse brain using MBP Polyclonal Antibody at dilution of 1:300.
Immunohistochemistry analysis of paraffin-embedded rat brain using MBP Polyclonal Antibody at dilution of 1:500.
