METTL3 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3580 |
货号：E-AB-63141
宿主: Rabbit
反应性: H,M,R
应用: WB,IF
Overview
| Synonyms | IME4,M6A,MT-A70,Spo8,hMETTL3,METTL3,METTL3抗体 |
| Swissprot | Q86U44 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant fusion protein of human METTL3 (NP_062826.2). |
| Application | WB(Detection kit: E-IR-R304),IF |
| Recommended dilution | WB,, 1:500-1:2000;IF,, 1:50-1:200; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus speckle. Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Background | This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. |
Images
Western blot analysis of extracts of various cell lines using METTL3 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of C6 cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
