Myeloperoxidase (MPO) Activity Assay Kit
髓过氧化物酶(MPO)比色法测试盒
| 规格： | / 50 assays(25 samples) / 100 assays(50 samples) |
| 价格： | / ￥220 / ￥350 |
货号：E-BC-K074-S
检测仪器及波长: 紫外-可见光分光光度计（460 nm）
样本类型: 血清;血浆;乳汁;动物组织;细胞
检测原理
髓过氧化物酶使过氧化氢还原成一种复合物，该复合物通过供氢体邻联茴香胺供氢后生成黄色产物，在460 nm处通过比色测定产物的生成量，从而计算出MPO的活力。
关键信息
| 指标简称 | MPO |
| 样本类型 | 血清;血浆;乳汁;动物组织;细胞 |
| 灵敏度 | 16.95 U/L |
| 检测范围 | 16.95-3349 U/L |
| 精密度 | 批间差：9.8%批内差：4.5% |
| 检测仪器及波长 | 分光光度计 |
| 特殊仪器 | 恒温水浴锅 |
| 保存条件 | 2-8℃ |
| 有效期 | 6个月 |
样本稀释倍数
在正式检测前，需选择2-3个预期差异大的样本稀释成不同浓度进行预实验，结合本试剂盒的检测范围（16.95-3349 U/L），选取最佳稀释倍数，进行正式批量实验。不同样本的稀释比例范围如下表（仅供参考）:
|
样本 |
稀释倍数 |
样本 |
稀释倍数 |
|
人血清 |
不稀释 |
5%小鼠脑组织 |
不稀释 |
|
人血浆 |
不稀释 |
5%大鼠心匀浆 |
不稀释 |
|
大鼠血清 |
不稀释 |
人乳汁 |
不稀释 |
注：稀释液为试剂二应用液。
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