p27 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70373
宿主: Rabbit
反应性: H
应用: IHC
Overview
| Synonyms | AA408329,AI843786,Cdki1b,CDKN 1B,CDKN 4,CDKN1B,CDKN4,CDN1B,Cyclin Dependent Kinase Inhibitor 1B,Cyclin dependent kinase inhibitor p27,Cyclin-dependent kinase inhibitor 1B (p27,Kip1),Cyclin-dependent kinase inhibitor 1B,Cyclin-dependent kinase inhibitor p27,Cyclin-dependent kinase inhibitor p27 Kip1,KIP 1,KIP1,MEN1B,MEN4,OTTHUMP00000195098,OTTHUMP00000195099,p27,p27 Kip1,P27-like cyclin-dependent kinase inhibitor,p27Kip1,p27抗体 |
| Swissprot | P46527,P46414,O08769 |
| Source | Rabbit |
| Reactivity | Human |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Human p27 KIP 1. |
| Application | IHC(Detection kit: E-IR-R213) |
| Recommended dilution | IHC,,1:300-1:1000 |
| Concentration | 500 μg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation. |
| Tissue specificity | Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.Highest expression level in pigmented layer of retina |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). |
Images
Immunohistochemistry analysis of paraffin-embedded human lung cancer using p27 Polyclonal Antibody at dilution of 1:400.
Immunohistochemistry analysis of paraffin-embedded human colon using p27 Polyclonal Antibody at dilution of 1:400.
Immunohistochemistry analysis of paraffin-embedded human breast using p27 Polyclonal Antibody at dilution of 1:400.
Immunohistochemistry analysis of paraffin-embedded human esophagi cancer using p27 Polyclonal Antibody at dilution of 1:400.
