p53RFP Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-15118
宿主: Rabbit
反应性: H,M
应用: WB,IHC
Overview
| Synonyms | bA528A10.3,E3 ubiquitin protein ligase RNF144B,E3 ubiquitin-protein ligase RNF144B,IBR domain containing 2,IBR domain containing protein 2,IBR domain-containing protein 2,IBRDC 2,IBRDC2,KIAA0161,MGC71786,OTTHUMP00000016079,OTTHUMP00000016080,OTTHUMP00000039360,p53 inducible RING finger protein,p53-inducible RING finger protein,p53RFP,PIR2,R144B,Ring finger 144B,RING finger protein 144B,RNF 144B,RNF144B,RNF144B抗体 |
| Swissprot | Q7Z419 |
| Source | Rabbit |
| Reactivity | Human,Mouse |
| Immunogen | Recombinant protein of human RNF144B |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:200-1:1000;IHC,,1:50-1:200; |
| Concentration | 0.4 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Mitochondrion membrane. Cytoplasm. Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation. |
| Tissue specificity | Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle. |
Images
Western Blot analysis of TM4 cell using p53RFP Polyclonal Antibody at dilution of 1:400
Immunohistochemistry of paraffin-embedded Human lung cancer using p53RFP Polyclonal Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Human cervical cancer using p53RFP Polyclonal Antibody at dilution of 1:50
