PCNA Monoclonal Antibody
| 规格： | / 20μL / 50μL / 100μL / 200μL |
| 价格： | / ￥219 / ￥499 / ￥800 / ￥1280 |
货号：E-AB-48013
宿主: Mouse
反应性: Z
应用: WB,IF,IHC
Overview
| Synonyms | ATLD2, cb16, Cyclin, DNA polymerase delta auxiliary protein, etID36690.10, fa28e03, fb36g03, HGCN8729, MGC8367, Mutagen-sensitive 209 protein, OTTHUMP00000030189, OTTHUMP00000030190, Pcna/cyclin, PCNAR, Polymerase delta accessory protein, Proliferating cell nuclear antigen, wu:fa28e03, wu:fb36g03 |
| Swissprot | P12004 |
| Source | Mouse |
| Reactivity | Zebrafish |
| Immunogen | Recombinant human PCNA protein expressed by E.coli |
| Application | WB(Detection kit: E-IR-R304),IF,IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:1000-2000; |
| Concentration | 2mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Nucleus |
| Clone No | 8B12 |
|---|---|
| Isotype | IgG1 |
| Purification | Protein A/G Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4 |
| Background | Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics. PCNA gene is often stably and constitutively expressed in most tissues and cells, it is commonly used as a loading control for western blot and other test. |
Images
Western blot of Zebrafish whole lysates with anti-PCNA monoclonal antibody at dilution of 1:1000.
Immunofluorescence of Zebrafish frozen section with anti-PCNA monoclonal antibodyat dilution of 1:100.
Immunohistochemistry of Zebrafish paraffin embedded section with anti-PCNA mcab at dilution of 1:100.
