Phospho-AKT1 (Ser473) Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥630 / ￥1510 / ￥2415 |
货号：E-AB-21336
宿主: Mouse
反应性: H
应用: WB,IHC-p
Overview
| Synonyms | AKT 1,AKT,AKT1,AKT1,MGC99656,PKB,PKB-ALPHA,PRKBA,Protein Kinase B Alpha,Protein kinase B,Proto-oncogene c-Akt,RAC Alpha,RAC,RAC-alpha serine/threonine-protein kinase,RAC-PK-alpha,Phospho-Akt (S473) Mouse (6F8)磷酸化抗体 |
| Swissprot | P31749 |
| Source | Mouse |
| Reactivity | Human |
| Immunogen | Synthetic Peptide of Phospho-Akt (Ser473) |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213) |
| Recommended dilution | WB 1:1000-2000, IHC 1:100-200 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. |
| Tissue specificity | Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. |
| Clone No | Clone:3B3 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. |
Images
Western Blot analysis of PC-3 cells using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:1000
Immunohistochemistry of paraffin-embedded Human lung carcinoma tissue using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using Phospho-AKT1 (Ser473) Monoclonal Antibody at dilution of 1:200
客户发表文献
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样品类型: Cell lysate
