Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥630 / ￥1510 / ￥2415 |
货号：E-AB-21121
宿主: Rabbit
反应性: H,M,R,Mk
应用: WB,IHC-p,IF
Overview
| Synonyms | PRKAA1,AMPK1,5'-AMP-activated protein kinase catalytic subunit alpha-1,AMPK subunit alpha-1,Acetyl-CoA carboxylase kinase,ACACA kinase,Hydroxymethylglutaryl-CoA reductase kinase,HMGCR kinase,Tau-protein kinase PRKAA1,PRKAA2,AMPK,,AMPKα1/2 (phospho Thr183/172)磷酸化抗体 |
| Swissprot | Q13131,P54646 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat,Monkey |
| Immunogen | Synthesized peptide derived from human AMPKα1/2 around the phosphorylation site of Thr183/172 |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300;IF,,1:50-1:200; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytosol,Nucleus,nuclear speck,nucleoplasm,nucleus,Plasma Membrane,apical plasma membrane,Other locations:cytoplasm,intracellular,nucleotide-activated protein kinase complex |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. |
Images
Western Blot analysis of various cells using Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded mouse brain using Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody at dilution of 1:50
Immunofluorescence analysis of Rat heart tissue using Phospho-AMPK alpha1/2 (Thr183/172) Polyclonal Antibody at dilution of 1:200
