PLEKHO1 Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-11086
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC
Overview
| Synonyms | C-Jun-binding protein,Casein kinase 2-interacting protein 1,CK2 interacting protein 1,CK2 interacting protein 1 HQ0024c protein,CK2-interacting protein 1,CKIP 1,CKIP-1,CKIP1,JBP,OC120,Osteoclast maturation associated gene 120 protein,Osteoclast maturation-associated gene 120 protein,PH domain-containing family O member 1,PKHO1,Pleckstrin homology domain containing family O member 1,Pleckstrin homology domain-containing family O member 1,PLEKHO1,PLEKHO1抗体 |
| Swissprot | Q53GL0 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein of human PLEKHO1 |
| Application | WB(Detection kit: E-IR-R304),IHC(Detection kit: E-IR-R213) |
| Recommended dilution | WB,,1:200-1:1000;IHC,,1:100-1:300; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cell membrane. Nucleus. Cytoplasm. Predominantly localized to the plasma membrane. In C2C12 cells, with the absence of growth factor, it is found in the nucleus. It rapidly translocates to the plasma membrane after insulin stimulation. In response to TNF, it translocates from the plasma membrane to the cytoplasm and then to the nucleus accompanied by cleavage by caspase-3. However, the subcellular location is highly dependent of the cell type, and this explains why it is found exclusively at the plasma membrane, in some type of cells. |
| Tissue specificity | Abundantly expressed in skeletal muscle and heart, moderately in kidney, liver, brain and placenta and sparingly in the pancreas and lung. Easily detectable in cancer cell lines such as MOLT-4, HEK293 and Jurkat cells. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.05% sodium azide, 50% glycerol, PH7.3 |
| Background | Pleckstrin homology domain-containing family O member 1 is a protein that in humans is encoded by thePLEKHO1 gene.Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP).May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2).Appears to target ATM to the plasma membrane.Appears to also inhibit tumor cell growth by inhibiting AKT-mediated cell-survival. |
Images
Western Blot analysis of Hela cell, Mouse muscle and kidney tissue using PLEKHO1 Polyclonal Antibody at dilution of 1:200
Immunohistochemistry of paraffin-embedded Human thyroid cancer using PLEKHO1 Polyclonal Antibody at dilution of 1:70
Immunohistochemistry of paraffin-embedded Human cervical cancer using PLEKHO1 Polyclonal Antibody at dilution of 1:70
