PYCARD Polyclonal Antibody
| 规格： | / 20μL / 60μL / 120μL / 200μL |
| 价格： | / ￥420 / ￥1010 / ￥1640 / ￥2365 |
货号：E-AB-30582
宿主: Rabbit
反应性: H,M,R
应用: WB,IHC-p,IF
Overview
| Synonyms | Apoptosis associated speck like protein containing a CARD,Apoptosis-associated speck-like protein containing a CARD,ASC,ASC,CARD 5,CARD5,Caspase recruitment domain containing protein 5,Caspase recruitment domain protein 5,Caspase recruitment domain-containing protein 5,hASC,MGC10332,PYCARD,PYD and CARD domain containing,PYD and CARD domain containing protein,PYD and CARD domain-containing protein,Target of methylation induced silencing 1,Target of methylation-induced silencing 1,TMS 1,TMS,TMS1,ASC抗体 |
| Swissprot | Q9ULZ3 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the N-terminal region of human ASC |
| Application | WB(Detection kit: E-IR-R304),IHC-p(Detection kit: E-IR-R213),IF |
| Recommended dilution | WB,,1:500-1:2000;IHC,,1:100-1:300;IF,,1:200-1:1000; |
| Concentration | 1mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. |
| Tissue specificity | Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
| Background | Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. |
