QuicKey Pro Human IL-8(Interleukin 8) ELISA Kit
QuicKey Pro-人白介素8(IL-8)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2400 / ￥1650 / ￥10800 |
货号：E-OSEL-H0014
反应性: 人
检测范围: 15.63~1000 pg/mL
灵敏度: 9.38 pg/mL
QuicKey Pro系列
与传统ELISA试剂盒相比，将实验时间缩短了至少1-2小时，同时提高了试剂盒的灵敏度，让客户可以获得更加精确的实验结果。该系列是Elabscience自主开发的新技术，旨在帮助客户以更为高效的方式进行科学研究。
用途
该试剂盒用于体外定量检测人血清、血浆或其他相关生物液体中IL-8浓度。
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗人 IL-8抗体包被于酶标板上，实验时加入样品（或标准品）和辣根过氧化物酶（HRP）标记的抗IL-8检测抗体，样本（或标准品中）的IL-8与包被抗体IL-8及HRP标记的抗IL-8抗体结合，形成包被抗体-IL-8-HRP标记的抗IL-8抗体的复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-8的浓度。
| 反应类型 | 双抗体夹心法 |
| 规格 | 96T |
| 反应时间 | 1.5h |
| 反应性 | 人 |
| 检测方法 | Colormetric |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 50μL |
| 样本类型 | 血清、血浆或其他相关生物液体 |
特异性
可检测样本中的人IL-8，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) |
54.94 | 152.77 | 425.52 | 55.10 | 154.08 | 436.69 |
| Standard
deviation |
3.28 | 6.97 | 16.13 | 3.07 | 8.67 | 14.89 |
| C V (%) | 5.97 | 4.56 | 3.79 | 5.58 | 5.63 | 3.41 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=8) | 91-103 | 97 |
| EDTA plasma (n=8) | 86-97 | 92 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | ||
|---|---|---|---|
| 1:2 | Range (%) | 95-106 | 86-98 |
| Average (%) | 101 | 91 | |
| 1:4 | Range (%) | 90-104 | 96-110 |
| Average (%) | 97 | 103 | |
| 1:8 | Range (%) | 80-92 | 85-101 |
| Average (%) | 86 | 92 | |
| 1:16 | Range (%) | 81-94 | 89-104 |
| Average (%) | 87 | 96 |
试剂盒组成及保存
未拆封的试剂盒可在2-8℃保存6个月；试剂盒组分开封后请按照下表中的条件分别保存。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA 酶标板 | Micro ELISA Plate (Dismountable) | 96T：8 孔×12 条 48T：8 孔×6 条 24T：8 孔×3 条 96T*5：5 块 96T酶标板 |
2-8℃, 可存放1个月 |
| 冻干标准品 | Reference Standard | 96T：2 支 48T/24T：1 支 96T*5：10 支 |
2-8℃, 复溶后当天使用 |
| 浓缩HRP酶结合抗体 (100×) | Concentrated HRP Linked Detection Ab(100×) | 96T：1支 60μL 48T/24T：1支 30μL 96T*5：5 支 60μL |
2-8℃(避光) |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 96T/48T/24T：1瓶 10mL 96T*5：5瓶 10mL |
2-8℃ |
| HRP酶结合抗体稀释液 | HRP Linked Ab Diluent | 96T/48T/24T：1瓶 7mL 96T*5：5瓶 7mL |
|
| 酶结合物稀释液 | Concentrated Wash Buffer(25×) | 96T/48T/24T：1瓶 30mL 96T*5：5瓶 30mL |
|
| 底物溶液(TMB) | Substrate Reagent | 96T/48T/24T：1瓶 10mL 96T*5：5瓶 10mL |
2-8℃(避光) |
| 反应终止液 | Stop Solution | 96T/48T/24T：1瓶 10mL 96T*5：5瓶 10mL |
2-8℃ |
| 封板覆膜 | Plate Sealer | 96T/48T/24T：5张 96T*5：25张 |
|
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1.酶标仪(450nm波长滤光片)
- 2.高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3.37℃恒温箱，双蒸水或去离子水
- 4.吸水纸
操作步骤
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