Rat Bcl-2(B-cell Lymphoma/Leukemia 2) ELISA Kit
大鼠B细胞淋巴瘤因子2(Bcl-2)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2600 / ￥1800 / ￥11050 |
货号：E-EL-R0096
反应性: 大鼠
检测范围: 0.16~10 ng/mL
灵敏度: 0.10 ng/mL
用途
该试剂盒用于体外定量检测大鼠serum, plasma and other biological fluids中Bcl-2浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗大鼠Bcl-2抗体包被于酶标板上，实验时样品或标准品中的大鼠Bcl-2会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗大鼠Bcl-2抗体和辣根过氧化物酶标记的亲和素。抗大鼠Bcl-2抗体与结合在包被抗体上的大鼠Bcl-2结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，Bcl-2浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中Bcl-2的浓度。
| 反应类型 | 夹心法 |
| 规格 | 96T |
| 反应时间 | 3.5h |
| 反应性 | 大鼠 |
| 检测方法 | Colormetric |
| 检测范围 | 0.16—10 ng/mL |
| 灵敏度 | 0.10 ng/mL |
| 样本体积 | 100μL |
| 样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的大鼠Bcl-2，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (ng/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 10 | 2.324 2.328 |
2.326 | 2.258 |
| 5 | 1.643 1.683 |
1.663 | 1.595 |
| 2.5 | 0.932 0.912 |
0.922 | 0.854 |
| 1.25 | 0.502 0.528 |
0.515 | 0.447 |
| 0.63 | 0.283 0.273 |
0.278 | 0.21 |
| 0.31 | 0.181 0.153 |
0.167 | 0.099 |
| 0.16 | 0.11 0.13 |
0.12 | 0.052 |
| 0 | 0.061 0.075 |
0.068 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) |
0.50 | 1.40 | 3.80 | 0.50 | 1.40 | 4.00 |
| Standard
deviation |
0.03 | 0.06 | 0.17 | 0.03 | 0.07 | 0.20 |
| C V (%) | 6.00 | 4.29 | 4.47 | 6.00 | 5.00 | 5.00 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 86-99 | 93 |
| EDTA plasma (n=5) | 86-98 | 91 |
| Cell culture media (n=5) | 93-108 | 99 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 88-99 | 89-106 | 98-111 |
| Average (%) | 94 | 97 | 105 | |
| 1:4 | Range (%) | 99-116 | 82-96 | 95-106 |
| Average (%) | 106 | 88 | 101 | |
| 1:8 | Range (%) | 94-108 | 87-98 | 95-110 |
| Average (%) | 102 | 93 | 101 | |
| 1:16 | Range (%) | 96-112 | 87-99 | 91-105 |
| Average (%) | 104 | 94 | 99 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃，可存放6个月 |
| 冻干标准品 | Reference Standard | 2 支 | |
| 浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
| 浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光)，可存放6个月 |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
| 生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱，双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
|
1.对应板孔中加入100μL标准品工作液或样本，37℃孵育90分钟 |
| 2.弃掉板内液体后，立即加入100μL生物素化抗体工作液，37℃孵育60分钟 | |
| 3.弃掉板内液体，洗板3次 | |
| 4.每孔加入100μL HRP酶结合物工作液，37℃孵育30分钟，弃掉板内液体，洗板5次 | |
| 5.每孔加入90μL底物溶液，37℃孵育15分钟 | |
| 6.每孔加入50μL终止液 | |
| 7.立即在450nm波长下读数，处理数据 |
客户发表文献
- 文献: Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities
样品类型: Hippocampal Tissue - 文献: Protocatechuic acid mitigates cadmium-induced neurotoxicity in rats: Role of oxidative stress, inflammation and apoptosis
样品类型: cortical - 文献: Irbesartan reprofiling for the amelioration of ethanol-induced gastric mucosal injury in rats: Role of inflammation, apoptosis, and autophagy
样品类型: gastric tissue - 文献: The efficacy of bone marrow-derived mesenchymal stem cells and/or erythropoietin in ameliorating kidney damage in gamma irradiated rats: Role of non-hematopoietic erythropoietin anti-apoptotic signaling
样品类型: Tissue homogenate - 文献: Nano-ivabradine averts behavioral anomalies in Huntington's disease rat model via modulating Rhes/m-tor pathway
样品类型: serum - 文献: Betulinic acid protects against cardiotoxicity of the organophosphorus pesticide chlorpyrifos by suppressing oxidative stress, inflammation, and apoptosis in rats
样品类型: heart - 文献: Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats
样品类型: Cardiac,Renal - 文献: Donepezil halts acetic acid-induced experimental colitis in rats and its associated cognitive impairment through regulating inflammatory/oxidative/apoptotic cascades: An add-on to its anti-dementia activity
样品类型: Colon - 文献: Nrf2/HO-1 Signaling Stimulation through Acetyl-11-Keto-Beta-Boswellic Acid (AKBA) Provides Neuroprotection in Ethidium Bromide-Induced Experimental Model of Multiple Sclerosis
样品类型: brain - 文献: 3-Indolepropionic acid upturned male reproductive function by reducing oxido-inflammatory responses and apoptosis along the hypothalamic-pituitary-gonadal axis of adult rats exposed to chlorpyrifos
样品类型: testes,epididymis,hypothalamus - 文献: Licorice Ameliorates Cisplatin-Induced Hepatotoxicity Through Antiapoptosis, Antioxidative Stress, Anti-Inflammation, and Acceleration of Metabolism
样品类型: Tissue homogenate - 文献: Antiapoptotic effect of taurine against NMDA-induced retinal excitotoxicity in rats
样品类型: Tissue homogenate - 文献: Neuroprotective Effect of Magnesium Acetyltaurate Against NMDA-Induced Excitotoxicity in Rat Retina
样品类型: Tissue homogenate - 文献: Sarcococca saligna Hydroalcoholic Extract Ameliorates Arthritis in Complete Freund’s Adjuvant-Induced Arthritic Rats via Modulation of Inflammatory Biomarkers and Suppression of Oxidative Stress Markers
样品类型: serum - 文献: Guggulsterone ameliorates ethidium bromide-induced experimental model of multiple sclerosis via restoration of behavioral, molecular, neurochemical and morphological alterations in rat brain
样品类型: Tissue homogenate(brain) - 文献: Beneficial effects of ferulic acid alone and in combination with insulin in streptozotocin induced diabetic neuropathy in Sprague Dawley rats
样品类型: Tissue homogenate - 文献: Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway
样品类型: heart tissue - 文献: Sarcococca saligna ameliorated D-galactose induced neurodegeneration through repression of neurodegenerative and oxidative stress biomarkers
样品类型: Brain tissue - 文献: Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats
样品类型: hippocampal tissue - 文献: Decrease in reproductive dysfunction using aflatoxin B1 exposure: a treatment with 3-indolepropionic acid in albino Wistar rat
样品类型: testicular,epididymal,hypothalamic - 文献: Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: insights into the role of adenosine A1 receptors
样品类型: retinal - 文献: Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway
样品类型: serum - 文献: Involvement of redox status and the nuclear-related factor 2 in protecting against cadmium-induced renal injury with Sana Makki (Cassia senna L.) pre-treatment in male rats
样品类型: Serum,Tissue homogenate - 文献: Sleep deprivation disrupts striatal anti-apoptotic responses in 6-hydroxy dopamine-lesioned parkinsonian rats
样品类型: Tissue homogenate - 文献: miR‑9 inhibition of neuronal apoptosis and expression levels of apoptosis genes Bcl‑2 and Bax in depression model rats through Notch pathway
样品类型: serum
