Rat VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit
大鼠血管内皮细胞生长因子A(VEGF-A)酶联免疫吸附测定试剂盒
| 规格： | / 96T / 48T / 96T*5 |
| 价格： | / ￥2400 / ￥1650 / ￥10200 |
货号：E-EL-R2603
反应性: 大鼠
检测范围: 31.25~2000 pg/mL
灵敏度: 18.75 pg/mL
用途
该试剂盒用于体外定量检测大鼠serum, plasma and other biological fluids中VEGF-A浓度
检测原理
本试剂盒采用双抗体夹心ELISA法。用抗大鼠VEGF-A抗体包被于酶标板上，实验时样品或标准品中的大鼠VEGF-A会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗大鼠VEGF-A抗体和辣根过氧化物酶标记的亲和素。抗大鼠VEGF-A抗体与结合在包被抗体上的大鼠VEGF-A结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，VEGF-A浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中VEGF-A的浓度。
| 反应类型 | 夹心法 |
| 规格 | 96T |
| 反应时间 | 3.5h |
| 反应性 | 大鼠 |
| 检测方法 | Colormetric |
| 检测范围 | 31.25—2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100μL |
| 样本类型 | serum, plasma and other biological fluids |
特异性
可检测样本中的大鼠VEGF-A，且与其它相关蛋白无明显交叉反应。
重复性
板内，板间变异系数均<10%。
典型数据
由于实验操作条件的不同（如操作者、移液技术、洗板技术和稳定条件等），标准曲线的OD值会有所差异。以下数据和曲线仅供参考，实验者需根据自己的实验建立标准曲线。
| (pg/mL) | O.D | Average | Corrected |
|---|---|---|---|
| 2000 | 2.33 2.366 |
2.348 | 2.264 |
| 1000 | 1.562 1.602 |
1.582 | 1.498 |
| 500 | 1.006 0.968 |
0.987 | 0.903 |
| 250 | 0.475 0.497 |
0.486 | 0.402 |
| 125 | 0.297 0.281 |
0.289 | 0.205 |
| 62.5 | 0.192 0.178 |
0.185 | 0.101 |
| 31.25 | 0.133 0.139 |
0.136 | 0.052 |
| 0 | 0.078 0.09 |
0.084 | -- |
精密度
板内精密度:低浓度样本,中浓度样本和高浓度样本分别在1块板子上检测20次。
板间精密度:低浓度样本,中浓度样本和高浓度样本分别在3块板子上检测20次。
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) |
97.90 | 170.39 | 948.99 | 89.62 | 158.20 | 968.59 |
| Standard
deviation |
5.02 | 9.25 | 46.60 | 5.65 | 7.26 | 37.97 |
| C V (%) | 5.13 | 5.43 | 4.91 | 6.30 | 4.59 | 3.92 |
回收率
分别往5个不同样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum (n=5) | 86-101 | 93 |
| EDTA plasma (n=5) | 90-102 | 95 |
| Cell culture media (n=5) | 89-101 | 95 |
线性
分别往5个样本中添加已知浓度的目标蛋白，做回收实验，得出回收率范围及平均回收率。将5个样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围及平均回收率。
| Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
|---|---|---|---|---|
| 1:2 | Range (%) | 89-102 | 86-98 | 96-112 |
| Average (%) | 95 | 93 | 103 | |
| 1:4 | Range (%) | 88-99 | 84-99 | 87-102 |
| Average (%) | 94 | 91 | 94 | |
| 1:8 | Range (%) | 94-107 | 83-96 | 85-99 |
| Average (%) | 100 | 88 | 91 | |
| 1:16 | Range (%) | 89-103 | 80-90 | 87-101 |
| Average (%) | 95 | 86 | 92 |
试剂盒组成及保存
未拆封的试剂盒可在4℃保存一周；如果一周以后才使用试剂盒，请拆开试剂盒按照下表中的条件分别保存各组分。
| 中文名称 | 英文名称 | 规格 | 保存条件 |
|---|---|---|---|
| ELISA酶标板(可拆卸) | Micro ELISA Plate(Dismountable) | 8 孔×12 条 | -20℃，可存放6个月 |
| 冻干标准品 | Reference Standard | 2 支 | |
| 浓缩生物素化抗体(100×) | Concentrated Biotinylated Detection Ab | 1支 120 μL | |
| 浓缩HRP酶结合物(100×) | Concentrated HRP Conjugate | 1支 120 μL | -20℃(避光)，可存放6个月 |
| 标准品&样品稀释液 | Reference Standard & Sample Diluent | 1瓶 20 mL | 4℃.可存放6个月 |
| 生物素化抗体稀释液 | Biotinylated Detection Ab Diluent | 1瓶 14 mL | |
| 酶结合物稀释液 | HRP Conjugate Diluent | 1瓶 14 mL | |
| 浓缩洗涤液(25×) | Concentrated Wash Buffer (25×) | 1瓶 30 mL | |
| 底物溶液(TMB) | Substrate Reagent | 1瓶 10 mL | 4℃(避光) |
| 反应终止液 | Stop Solution | 1瓶 10 mL | 4℃ |
| 封板覆膜 | Plate Sealer | 5 张 | |
| 产品说明书 | Product Description | 1 份 | |
| 质检报告 | Certificate of Analysis | 1 份 |
说明：所有试剂瓶盖须旋紧以防止蒸发和微生物的污染。
试剂体积以实际发货版说明书为准。相关试剂在分装时会比标签上标明的体积稍多一些，请在使用时量取而非直接倒出。
试验所需自备物品
- 1. 酶标仪(450nm波长滤光片)
- 2. 高精度移液器，EP管及一次性吸头：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL
- 3. 37℃恒温箱，双蒸水或去离子水
- 4. 吸水纸
操作步骤
|
|
1.对应板孔中加入100μL标准品工作液或样本，37℃孵育90分钟 |
| 2.弃掉板内液体后，立即加入100μL生物素化抗体工作液，37℃孵育60分钟 | |
| 3.弃掉板内液体，洗板3次 | |
| 4.每孔加入100μL HRP酶结合物工作液，37℃孵育30分钟，弃掉板内液体，洗板5次 | |
| 5.每孔加入90μL底物溶液，37℃孵育15分钟 | |
| 6.每孔加入50μL终止液 | |
| 7.立即在450nm波长下读数，处理数据 |
客户发表文献
- 文献: Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair
样品类型: Cell culture supernatant - 文献: MRI‐Visible siRNA Nanomedicine Directing Neuronal Differentiation of Neural Stem Cells in Stroke
样品类型: neural stem cells (NSCs) - 文献: Rock Climbing-Inspired Electrohydrodynamic Cryoprinting of Micropatterned Porous Fiber Scaffolds with Improved MSC Therapy for Wound Healing
- 文献: Spatiotemporal regulation of the bone immune microenvironment via dam-like biphasic bionic periosteum for bone regeneration
样品类型: BMSCs - 文献: Exosome-mimetics as an engineered gene-activated matrix induces in-situ vascularized osteogenesis
样品类型: Cell culture supernatant - 文献: Bone marrow mesenchymal stem cells loaded into hydrogel/nanofiber composite scaffolds ameliorate ischemic brain injury
样品类型: bone marrow mesenchymal stem cells (BMSCs) - 文献: Mild photothermal effect of titania nanotubes array as a promising solution for peri-implantitis
样品类型: EPCs - 文献: The Role of Neuregulin-1 in Steatotic and Non-Steatotic Liver Transplantation from Brain-Dead Donors
样品类型: liver tissue - 文献: SENP6-Mediated deSUMOylation of VEGFR2 Enhances Its Cell Membrane Transport in Angiogenesis
样品类型: retinal microvascular pericytes (RMPs) - 文献: Potential Therapeutic Effects of Mi-Jian-Chang-Pu Decoction on Neurochemical and Metabolic Changes of Cerebral Ischemia-Reperfusion Injury in Rats
样品类型: brain - 文献: Integration of transdermal chemistry and network pharmacology to decipher the mechanism of ShexiangZhuifeng analgesic plaster to treat rheumatoid arthritis
样品类型: serum - 文献: Attenuated effect of zinc gluconate on oxidative stress, inflammation, and angiogenic imbalance in pre-eclampsia rats
样品类型: serum - 文献: Co-transplantation with adipose-derived cells to improve parathyroid transplantation in a mice model
样品类型: Plasma - 文献: Fabrication of In Situ Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model
样品类型: Tissue homogenate,Serum - 文献: Integration of synthetic and natural derivatives revives the therapeutic potential of temozolomide against glioma- an in vitro and in vivo perspective
样品类型: C6 cell,U-87 MG cell,LN229 cell - 文献: MicroRNA-142-3p suppresses endometriosis by regulating KLF9-mediated autophagy in vitro and in vivo
样品类型: Serum,Cell culture medium - 文献: Rivaroxaban Modulates TLR4/Myd88/NF-Kβ Signaling Pathway in a Dose-Dependent Manner With Suppression of Oxidative Stress and Inflammation in an Experimental Model of Depression
样品类型: hippocampal tissue - 文献: Exosomes Derived From Mesenchymal Stem Cells Pretreated With Ischemic Rat Heart Extracts Promote Angiogenesis via the Delivery of DMBT1:
样品类型: heart tissue - 文献: hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway
样品类型: serum,NR8383 cell - 文献: Investigation on the Q-markers of Bushen Huoxue Prescriptions for DR treatment based on chemometric methods and spectrum-effect relationship
样品类型: eyes - 文献: A nano phototheranostic approach of toluidine blue conjugated gold silver core shells mediated photodynamic therapy to treat diabetic foot ulcer
样品类型: serum - 文献: The effect of gap junction-mediated transfer of miR-200b on osteogenesis and angiogenesis in a co-culture of MSCs and HUVECs
样品类型: Tissue homogenate - 文献: Amelioration of testosterone-induced benign prostatic hyperplasia using febuxostat in rats: The role of VEGF/TGFβ and iNOS/COX-2
样品类型: Serum - 文献: Iron oxide nanoparticles promote the migration of mesenchymal stem cells to injury sites
样品类型: Cell culture supernatant - 文献: Quinone-rich fraction of Ardisia crispa (Thunb.) A. DC roots alters angiogenic cascade in collagen-induced arthritis
样品类型: ankle - 文献: Anti-inflammatory potential of liraglutide, a glucagon-like peptide-1 receptor agonist, in rats with peripheral acute inflammation
样品类型: paw tissue - 文献: Curative effect and mechanisms of Radix Arnebiae oil on burn wound healing in rats
样品类型: burn wound tissue - 文献: Inhibiting expression of Cxcl9 promotes angiogenesis in MSCs-HUVECs co-culture
样品类型: Cell lysate - 文献: Expression and Possible Role of Silent Mating Type Information Regulation 2 Homolog 1 in Post-necrotizing Enterocolitis Stricture in vivo and in vitro
样品类型: IEC-6 cell - 文献: Sphingosine kinase 1/sphingosine 1-phosphate/sphingosine 1-phosphate receptor 1 pathway: A novel target of geniposide to inhibit angiogenesis
样品类型: Serum - 文献: The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models
样品类型: VEGF solution after immobilization - 文献: Sildenafil ameliorates Alzheimer disease via the modulation of vascular endothelial growth factor and vascular cell adhesion molecule-1 in rats:
样品类型: Tissue homogenate - 文献: PTEN/PI3K/VEGF signaling pathway involved in the protective effect of xanthine oxidase inhibitor febuxostat against endometrial hyperplasia in rats:
样品类型: Tissue homogenate - 文献: Electroacupuncture pretreatment promotes angiogenesis via hypoxia-inducible factor 1α and vascular endothelial growth factor in a rat model of chronic myocardial ischemia:
样品类型: Serum - 文献: Combination of mild therapeutic hypothermia and adipose-derived stem cells for ischemic brain injury
样品类型: serum - 文献: Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer
样品类型: gastric tissue. - 文献: Molecular Mechanism of Traditional Chinese Ointment of Xuzhou Qufu Shengji in Infected Wounds
样品类型: skin - 文献: The Effect of Waterpipe Tobacco Smoking on Bone Healing Following Femoral Fractures in Male Rats
样品类型: serum - 文献: Impact of repeated intravenous infusions of umbilical cord–derived versus bone marrow–derived mesenchymal stem cells on angiogenesis in a pregnant experimentally induced deep venous thrombosis rat model
样品类型: serum - 文献: Protective effects of essential oil from Fructus Alpiniae zerumbet on retinal Müller gliosis via the PPAR-γ-p-CREB signaling pathway
样品类型: Serum,Cell culture medium - 文献: Comparison of the effect of topical bevacizumab and sorafenib in experimental corneal neovascularization
样品类型: corneal tissue - 文献: MMP‑sensitive PEG hydrogel modified with RGD promotes bFGF, VEGF and EPC‑mediated angiogenesis
样品类型: EPCs cells - 文献: Citrus hystrix leaf extract attenuated diabetic‐cataract in STZ‐rats
样品类型: serum - 文献: Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM‐1 and VEGF‐A
样品类型: Serum - 文献: The effect of bay leaf extract (Syzygium polyanthum) on vascular endothelial growth factor (VEGF) and CD31 (PECAM-1) expression in acute coronary syndrome.
样品类型: blood - 文献: Expression and possible role of Smad3 in postnecrotizing enterocolitis stricture
样品类型: IEC-6 cell - 文献: Effects of mesenchymal stem cell and amnion membrane transfer on prevention of pericardial adhesions
样品类型: serum - 文献: The Inhibitory Effects of PSS-Loaded Nanoparticles on the Dysfunction of Cardiac Microvascular Endothelia in Rats with Diabetic Cardiomyopathy
样品类型: Serum
