RBFOX3 Polyclonal Antibody
| 规格： | / 60μL / 120μL / 200μL |
| 价格： | / ￥1380 / ￥2220 / ￥3300 |
货号：E-AB-70331
宿主: Rabbit
反应性: H,M,R
应用: WB
Overview
| Synonyms | FLJ56884,FLJ58356,Fox-1 homolog C,fox1 homolog C,Fox3,FOX3NeuN,hexaribonucleotide binding protein 3,HRNBP3,NEUN,neuronal nuclei,Rbfox3,RFOX3,RNA binding protein fox-1 homolog 3,RNA binding protein,fox 1 homolog (C. elegans) 3,RBFOX3抗体 |
| Swissprot | A6NFN3,Q8BIF2 |
| Source | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Recombinant protein corresponding to Mouse NEUN |
| Application | WB(Detection kit: E-IR-R304) |
| Recommended dilution | WB,,1:500-1:2000; |
| Concentration | 1 mg/mL |
| Clonality | Polyclonal |
Properties
| Cellular localization | Cytoplasm, Nucleus |
| Tissue specificity | Highest expression level in cerebellar vermis |
| Isotype | IgG |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
| Background | Immunoprecipitation and mass spectrometry of the two major NeuN species at 45–50 kDa identified both as the RNA binding protein Rbfox3 (a member of the Fox family of alternative splicing factors), confirming and extending the identification of the 45 kDa band as Rbfox3. Mapping of the anti-NeuN reactive epitopes in both R3hdm2 and Rbfox3 reveals a common proline- and glutamine-rich domain that lies at the N-terminus of the Rbfox3 protein. Nuclear Rbfox3 isoforms can also enhance the inclusion of cryptic exons in the Rbfox2 mRNA, resulting in nonsense-mediated decay of the message, thereby contributing to the negative regulation of Rbfox2 by Rbfox3 through a novel mechanism. |
