Recombinant GAPDH Monoclonal Antibody
| 规格： | / 25μL / 100μL |
| 价格： | / ￥300 / ￥780 |
货号：AN004430L
货期：咨询
宿主: Rabbit
反应性: H;M;R
应用: WB;IHC;IF
Overview
| Synonyms | aging associated gene 9 protein;BARS-38;cb609;G3PDH;GAPD;KNC-NDS6;OCAS;p38 component |
| Swissprot | P16858 |
| Source | Rabbit |
| Reactivity | Human;Mouse;Rat |
| Immunogen | Recombinant protein within Mouse GAPDH |
| Application | WB(Detection kit: E-IR-R304);IHC(Detection kit: E-IR-R213);IF |
| Recommended dilution | WB,,1:20000-1:100000;IHC,,1:2000-1:4000;IF,,1:2000-1:4000 |
| Concentration | 1mg/mL |
| Clonality | Rabbit Monoclonal |
Properties
| Clone No | Da-20B8 |
|---|---|
| Isotype | IgG |
| Purification | Protein A Affinity Purified |
| Conjugation | Unconjugated |
| Storage instructions | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Protected from prolonged exposure to light. |
| Storage buffer | TBS with 0.05% sodium azide, 0.05% protective protein and 40% glycerol, pH7.4 |
| Background | GAPDH is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells,it is commonly used as a loading control for western blot and as a control for qPCR. |
Images
Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1:A431 cell lysate,Lane 2:Caco-2 cell lysate, Lane 3:Jurkat cell lysate, Lane 4:K562 cell lysate, Lane 5:HeLa cell lysate, Lane 6:PC-3 cell lysate, Lane 7:Ramos cell lysate, Lane 8:TF-1 cell lysate, Lane 9:U20-S cell lysate, Lane 10:HL-60 cell lysate, Lane 11:HT-29 cell lysate, Lane 12:Raji cell lysate, Lane 13:HepG2 cell lysate
Western blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1: 160000. lane 1: mouse heart tissue lysate, lane 2: mouse liver tissue lysate, lane 3: mouse uterus tissue lysate, lane 4: mouse lung tissue lysate, lane 5: mouse kidney tissue lysate, lane 6: mouse brain tissue lysate, lane 7: mouse stomach tissue lysate
Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1:C6 cell lysate, Lane 2:PC-12 cell lysate, Lane 3:Rat heart tissue lysate, Lane 4:Rat liver tissue lysate, Lane 5:Rat spleen tissue lysate, Lane 6:Rat brain tissue lysate, Lane 7:Rat stomach tissue lysate, Lane 8:Rat testis tissue lysate, Lane 9:Rat thymus tissue lysate, Lane 10:Rat skeletal muscle tissue lysate
Immunohistochemistry of paraffin-embedded human lung cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
Immunohistochemistry of paraffin-embedded rat ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.
