SMAD3 Monoclonal Antibody
| 规格： | / 20μL / 60μL / 120μL |
| 价格： | / ￥580 / ￥1420 / ￥2310 |
货号：E-AB-22241
宿主: Mouse
反应性: H,M,R
应用: WB,IF
Overview
| Synonyms | DKFZP586N0721,DKFZp686J10186,hMAD 3,hMAD-3,hSMAD3,HSPC193,HST17436,JV15 2,JV15-2,JV152,LDS1C,LDS3,MAD (mothers against decapentaplegic Drosophila) homolog 3,MAD homolog 3,Mad homolog JV15 2,Mad protein homolog,MAD,mothers against decapentaplegic homolog 3,Mad3,MADH 3,MADH3,MGC60396,Mothers against decapentaplegic homolog 3,Mothers against DPP homolog 3,SMA and MAD related protein 3,SMAD 3,SMAD,SMAD family member 3,SMAD,mothers against DPP homolog 3,Smad3,SMAD3,Smad3 Mouse (4C9)抗体 |
| Swissprot | P84022 |
| Source | Mouse |
| Reactivity | Human,Mouse,Rat |
| Immunogen | Synthetic Peptide of Smad3 |
| Application | WB(Detection kit: E-IR-R304),IF |
| Recommended dilution | IHC,,1:100-200 |
| Concentration | 1mg/mL |
| Clonality | Monoclonal |
Properties
| Cellular localization | Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). |
| Clone No | Clone:4C9 |
|---|---|
| Isotype | IgG |
| Purification | Protein A purification |
| Storage instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage buffer | PBS with 0.02% sodium azide,0.5% BSA and 50% glycerol pH 7.4. |
| Background | Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
